Local bradyrhizobia promote plant growth on both maize and legume

N'Zoué Angèle, Moulin Lionel, Prin Yves, Willems Anne, Laguerre Gisèle, Moudiongui Adako, Kouamé Christophe, Galiana Antoine, Dreyfus Bernard, De Lajudie Philippe. 2008. Local bradyrhizobia promote plant growth on both maize and legume. In : 8th European Nitrogen Fixation Conference, August 30 - September 3, 2008, Gent, Belgium. s.l. : s.n., Résumé, 1 p. European Nitrogen Fixation Conference. 8, Ghent, Belgique, 30 August 2008/3 September 2008.

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Abstract : In Ivory Coast (West Africa) food crops are widely produced through a legume-cereal-tuber intermixed culture system. To assess the role and genetic diversity of rhizobial bacteria in this agronomic system, we isolated 74 rhizobial strains from root nodules of groundnut, soybean, and cowpea plants grown as intermixed cultures in fields all around the country. They all belonged to the Bradyrhizobium species (16S rDNA sequencing) with a large genetic diversity (16S-23S rDNA intertranscribed spacer [ITS] sequencing). They formed 17 clusters, some corresponding to B. japonicum, B. elkanii, B. yuanmingense, some to Bradyrhizobium spp. reference genospecies Ia, IV, V, VIII, XI [1], and six separate groups. We further performed MultiLocus Sequence analysis on five housekeeping genes (gnlII, recA, dnaK, rpoB, and atpD), using several phylogenetic methods (MP, ML, Bayesian), and obtained a consensus phylogenetic tree. Greenhouse tests were performed to screen the new strains for (i) their nitrogen-fixing potential on groundnut, soybean, and cowpea, and (ii) their plant growth promotion capacity on maize. Eight weeks after inoculation, plants were surveyed for their nodule numbers and dry weights, aerial part and root dry weight, leaf color, and ARA. Statistical analysis show that all strains (100%) are efficient on cowpea, 26% on groundnut (separate genospecies clusters 3, 8, 9, and 10), and 27% on soybean (B. japonicum cl. I, Ia, B. elkanii, cl. II, B. yuanmingense). Six strains (STM3040, STM3080, STM3078, STM3088, STM3070, and STM3079) were selected for their growth promotion on both legumes and cereal. Biochemicals tests showed ammonium production in STM3078, siderophores production in STM3080, STM3078, and STM3088. No AIA production nor phosphate solubilization were detected. Further investigations for 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, ACC gene sequencing, and endophytic functions are in progress and will be discussed. (Texte intégral)

Mots-clés Agrovoc : Zea mays, légume

Mots-clés géographiques Agrovoc : Côte d'Ivoire

Classification Agris : P34 - Soil biology
F01 - Crops
F62 - Plant physiology - Growth and development

Auteurs et affiliations

  • N'Zoué Angèle, CIRAD-BIOS-UMR LSTM (FRA)
  • Moulin Lionel, IRD (FRA) ORCID: 0000-0001-9068-6912
  • Prin Yves, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0002-3706-0045
  • Willems Anne, Univeriteit Gent (BEL)
  • Laguerre Gisèle, INRA (FRA)
  • Moudiongui Adako, CNRA (CIV)
  • Kouamé Christophe, CNRA (CIV)
  • Galiana Antoine, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0002-5293-5049
  • Dreyfus Bernard, IRD (FRA)
  • De Lajudie Philippe, IRD (FRA)

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