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Revision of ploidy levels of Dioscorea alata polyploid species by cytogenetic and microsatellite segregation analysis

Arnau Gemma, Némorin Alice, Maledon Erick, Abraham Kuttolamadathil. 2009. Revision of ploidy levels of Dioscorea alata polyploid species by cytogenetic and microsatellite segregation analysis. In : International Conference on Polyploidy, Hybridization and Biodiversity, May 17 - 20, 2009, Saint-Malo, France, program and abstracts. Université de Rennes 1. Rennes : Université de Rennes 1, Résumé, 153. International Conference on Polyploidy, Hybridization and Biodiversity, Saint-Malo, France, 17 Mai 2009/20 Mai 2009.

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Résumé : Dioscorea alata is a polyploid species with several ploidy levels and its basic chromosome number has been considered by most authors to be x = 10. Standard chromosome counting and flow cytometry analysis were used to determine the chromosome number of 110 D. alata accessions of the CIRAD germplasm collection. The results revealed that 76% of accessions have 2n = 40 chromosomes, 7% have 2n = 60 chromosomes and 17% have 2n = 80 chromosomes. Progenies were produced from 2n = 40 types of D. alata and the segregation patterns of six microsatellite markers in four different progenies were analysed. The Bayesian method was used to test for diploid versus tetraploid (allo- and autotetraploid) modes of inheritance. The results provided the genetic evidence to establish the diploidy of plants with 2n = 40 chromosomes and to support the hypothesis that plants with 2n = 40, 60 and 80 chromosomes are diploids, triploids and tetraploids, respectively, and that the basic chromosome number of D. alata is x = 20. The findings obtained in the present study are significant for effective breeding programs, genetic diversity analysis and elucidation of the phylogeny and the species origin of D. alata. (Texte intégral)

Mots-clés Agrovoc : Dioscorea alata

Mots-clés géographiques Agrovoc : Inde, Guadeloupe, France

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

  • Arnau Gemma, CIRAD-BIOS-UPR Multiplication végétative (GLP) ORCID: 0000-0003-0714-6830
  • Némorin Alice, CIRAD-BIOS-UPR Multiplication végétative (GLP)
  • Maledon Erick, CIRAD-BIOS-UPR Multiplication végétative (GLP)
  • Abraham Kuttolamadathil, CTCRI (IND)

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Source : Cirad - Agritrop (https://agritrop.cirad.fr/550363/)

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