Capripoxvirus G-protein-coupled chemokine receptor : a host-range gene suitable for virus animal origin discrimination

Le Goff Christian, Euloge Lamien Charles, Fakhfakh Emna, Chadeyras Amélie, Aba-Adulugba Elexpeter, Libeau Geneviève, Tuppurainen Eeva, Wallace David B., Adam Tajelser, Silber Roland, Gulyaz Vely, Madani Hafsa, Caufour Philippe, Hammami Salah, Diallo Adama, Albina Emmanuel. 2009. Capripoxvirus G-protein-coupled chemokine receptor : a host-range gene suitable for virus animal origin discrimination. Journal of General Virology, 90 : pp. 1967-1977.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : VIROLOGY

Abstract : The genus Capripoxvirus within the family Poxviridae comprises three closely related viruses, namely goat pox, sheep pox and lumpy skin disease viruses. This nomenclature is based on the animal species from which the virus was first isolated, respectively, goat, sheep and cattle. Since capripoxviruses are serologically identical, their specific identification relies exclusively on the use of molecular tools. We describe here the suitability of the G-protein-coupled chemokine receptor (GPCR) gene for use in host-range grouping of capripoxviruses. The analysis of 58 capripoxviruses showed three tight genetic clusters consisting of goat pox, sheep pox and lumpy skin disease viruses. However, a few discrepancies exist with the classical virus-host origin nomenclature: a virus isolated from sheep is grouped in the goat poxvirus clade and vice versa. Intra-group diversity was further observed for the goat pox and lumpy skin disease virus isolates. Despite the presence of nine vaccine strains, no genetic determinants of virulence were identified on the GPCR gene. For sheep poxviruses, the addition or deletion of 21 nucleic acids (7 aa) was consistently observed in the 59 terminal part of the gene. Specific signatures for each cluster were also identified. Prediction of the capripoxvirus GPCR topology, and its comparison with other known mammalian GPCRs and viral homologues, revealed not only a classical GPCR profile in the last three-quarters of the protein but also unique features such as a longer N-terminal end with a proximal hydrophobic a-helix and a shorter serine-rich C-tail. (Résumé d'auteur)

Mots-clés Agrovoc : Capripoxvirus, Ovin, Caprin, Bovin

Mots-clés géographiques Agrovoc : Asie, Afrique

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Le Goff Christian, CIRAD-BIOS-UMR CMAEE (FRA)
  • Euloge Lamien Charles, AIEA (AUT)
  • Fakhfakh Emna, Institut de recherche vétérinaire (TUN)
  • Chadeyras Amélie
  • Aba-Adulugba Elexpeter, NVRI (NGA)
  • Libeau Geneviève
  • Tuppurainen Eeva, Institute of Animal Health (GBR)
  • Wallace David B., ARC (ZAF)
  • Adam Tajelser, Central Veterinary Research Laboratory (SDN)
  • Silber Roland, Institute for Veterinary Disease Control (AUT)
  • Gulyaz Vely, Pendik Veterinary Control and Research Institute (TUR)
  • Madani Hafsa, Institut national de la médecine vétérinaire (DZA)
  • Caufour Philippe, CIRAD-BIOS-UMR CMAEE (ETH)
  • Hammami Salah, IRVT (TUN)
  • Diallo Adama, CIRAD-BIOS-UMR CMAEE (ITA)
  • Albina Emmanuel, CIRAD-BIOS-UMR CMAEE (FRA)

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