Novos marcadores para fins de mapeamento e localizaçao de QTLs a partir de PCR-RFLP de genes do genoma café brasileiro : [Preprint]

Colombo Carlos Augusto, Yamamoto Paula Yuri, Ramos Luis Carlos S., Mazzafera Paulo, Gallo Paulo Boller, Vilella Otavia T., Lannes Sérgio Dias, Pot David, et al.. 2009. Novos marcadores para fins de mapeamento e localizaçao de QTLs a partir de PCR-RFLP de genes do genoma café brasileiro : [Preprint]. In : VI Simposio de Pesquisa dos Cafés do Brasil, 02 a 05 junho de 2009. s.l. : s.n., 5 p. Simposio de Pesquisa dos Cafés do Brasil. 6, Vitoria, Brésil, 2 Juin 2009/5 Juin 2009.

Titre anglais : New markers to mapping and location of QTLs from the brazilian coffee genome based on PCR-RFLP

Résumé : The genetic mapping strategy is very targeted for breeding and its importance is growing with the emergence of SNPs or INDELs markers, facilitated by the genome project, especially for ESTs. Thus, in silico analysis of polymorphic SNPs in sequences related to cup quality and derive from C.arabica and C.canephora were analyzed and the polymorphisms between the two species validated for six genes (four proteases and two sucrose) from laboratory studies using PCR-RFLP technique. Thus, after confirmation of polymorphism in both parental species, 90 F2 plants derived from self an interspecific hybrid of Coffea arabica and C. canephora 4x were genotyped. After obtaining the amplified, they were digested with various restriction enzymes of four bases (Alu I, DDE I, Eco RI, Hae III, Mse I and Msp, Fnu DII, Taq I, Scr FI). Of the six genes analyzed, four of them showed 3:1 segregation in the F2 population (Cysteine8, Cysteine 5, [bêta]-Fructosidase and sucrose phosphate synthase), demonstrating the use of them for mapping.

Mots-clés Agrovoc : Coffea arabica, Coffea canephora

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Colombo Carlos Augusto, IAC [Instituto Agronomico] (BRA)
• Yamamoto Paula Yuri, IAC [Instituto Agronomico] (BRA)
• Ramos Luis Carlos S., IAC [Instituto Agronomico] (BRA)
• Mazzafera Paulo, UNICAMP (BRA)
• Gallo Paulo Boller, APTA (BRA)
• Vilella Otavia T., IAC [Instituto Agronomico] (BRA)
• Lannes Sérgio Dias, IAPAR (BRA)
• Pot David, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0001-6144-8448
• et al.

Source : Cirad - Agritrop (https://agritrop.cirad.fr/551303/)

Métriques

[ Page générée et mise en cache le 2024-03-23 ]