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WAKomics : large-scale functional analysis of the WAK genes involved in the rice Magnaporthe grisea interaction

Delteil Amandine, Alart E., Morel Jean-Benoit. 2009. WAKomics : large-scale functional analysis of the WAK genes involved in the rice Magnaporthe grisea interaction. In : Abstracts of the XIV International Congress on Molecular Plant-Microbe Interactions, July 19-23, 2009, Quebec City, Canada. IS-MPMI. Saint-Paul : IS-MPMI, Résumé, 1 p. International Congress on Molecular Plant-Microbe Interactions. 14, Québec, Canada, 19 July 2009/23 July 2009.

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Abstract : Wall-associated protein kinases (WAKs) are a new group of receptor-like kinases (RLKs) recently identified in Arabidopsis and in rice. A gene responsible for the quantitative resistance to Fusarium oxysporum in Arabidopsis, RFO1, encodes a WAK-like protein (WAKL22). This finding suggests an important role for some WAKs in disease resistance. The first WAK in rice was recently identified as OsWAK1. The induction and overexpression of this gene in rice led to enhanced resistance to rice blast. Furthermore, WAKs also seem to interact with a wide range of proteins involved in cellwall composition, phosphorylation or transcription processes. This is functional evidence that WAKs are novel RLKs playing an important role in plant disease resistance. Microarray data reveal differential expression of some WAKs in rice leaves infected with Magnaporthe grisea. This set of WAKs was analysed by real time PCR in a time course experiment and confirmed the microarray results. This analysis also revealed differential expression for some WAKs very early after infection (4, 6 or 8 hours post inoculation), suggesting an early role in pathogen recognition. To confirm a role of these WAKs in rice blast resistance, Knock-Out and transgenic rice lines with constitutive expression of a subset of WAKs were produced. Kinase assays, localisation and molecular interactions using a reporter system are also planned. Preliminary data supporting the role of this gene family in disease resistance in rice will be presented. (Texte intégral)

Classification Agris : F30 - Plant genetics and breeding
H20 - Plant diseases

Auteurs et affiliations

  • Delteil Amandine, CIRAD-BIOS-UMR BGPI (FRA)
  • Alart E., Montpellier SupAgro (FRA)
  • Morel Jean-Benoit, INRA (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/552688/)

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