CD62L defines a subset of pathogen-specific bovine CD4 with central memory cell characteristics

Totté Philippe, Duperray Christophe, Dedieu Laurence. 2010. CD62L defines a subset of pathogen-specific bovine CD4 with central memory cell characteristics. Developmental and Comparative Immunology, 34 (2) : pp. 177-182.

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Quartile : Outlier, Sujet : ZOOLOGY / Quartile : Q2, Sujet : IMMUNOLOGY

Abstract : Central memory T cells (Tcm) have not previously been characterized in cattle and any other ruminant species. Here we described two phenotypically and functionally different subsets of pathogen-specific memory CD4+ T cells in cattle that survived infection with Mycoplasma mycoides subsp. mycoides small colony (MmmSC). The first subset is CD45RO+CD45R_CD62L_ and comprises two thirds of IFN-g producing CD4+ T cells after MmmSC recall stimulation. The second is CD45RO+CD45R_CD62L+ and represents the majority of proliferating CD4+ T cells after 7 days of stimulation. Cell sorting experiments confirmed that both CD4+CD62L+ and CD4+CD62L_ subsets are present in vivo and proliferate independently in recall responses to MmmSC. In addition, MmmSC stimulation strongly decreased CCR7 and increased CCR5 transcripts levels in CD4+CD62L_ cells whereas CD4+CD62L+ were only slightly affected. High levels of recall proliferation but low IFN-g production, together with the capacity to preferentially migrate through the lymph nodes (i.e., expression of CD62L and CCR7), are characteristics of Tcm, in humans and mice. Tcm are associated with long-term protective immunity and a privileged target for vaccine development. Our results demonstrate the existence of Tcm in cattle and suggest that CD62L may serve as a marker to monitor Tcm in infections and vaccine development studies in ruminant. (Résumé d'auteur)

Mots-clés Agrovoc : Mycoplasma mycoides, Ruminant, Bovin, Cellule, Relation hôte pathogène, Prolifération des tissus, Cytologie, Épidémiologie, Contrôle de maladies

Mots-clés complémentaires : Cellule T, Marqueur moléculaire

Classification Agris : L73 - Animal diseases
L52 - Animal physiology - Growth and development

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Totté Philippe, CIRAD-BIOS-UMR CMAEE (FRA)
  • Duperray Christophe, INSERM (FRA)
  • Dedieu Laurence, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0002-6492-3002

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