An OmpA family outer membrane protein is required for both disease symptom development and sugarcane stalk colonization by Xanthomonas albilineans

Rott Philippe, Fleites Laura, Marlow Gary C., Royer Monique, Gabriel Dean W.. 2009. An OmpA family outer membrane protein is required for both disease symptom development and sugarcane stalk colonization by Xanthomonas albilineans. Phytopathology, 99 (6S), Résumé, S110-S111 2009 APS Annual Meeting, Portland, États-Unis, 1 August 2009/5 August 2009.

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Quartile : Q2, Sujet : PLANT SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Xanthomonas albilineans (Xa) is a systemic, xylem-invading pathogen that causes sugarcane leaf scald. Leaf symptoms vary from a single, white, narrow, Vol. 99, No. 6 (Supplement), 2009 S111 sharply defined stripe to complete wilting and necrosis of infected leaves, leading to plant death. Xa produces the toxin albicidin that blocks chloroplast differentiation, resulting in disease symptoms. Albicidin is the only previously known pathogenicity factor in Xa, yet albicidin-deficient mutant strains are still able to efficiently colonize sugarcane. We used Tn5 (transposome) mutagenesis in an attempt to identify additional Xa pathogenicity factors. Sugarcane cultivar CP80-1743, moderately susceptible to leaf scald, was inoculated by the decapitation method with 780 independently derived Tn5 insertions in Florida strain XaFL07-1. Leaf scald symptoms were recorded on emerging leaves one month after inoculation, and stalk colonization by the pathogen was determined two months after inoculation. In addition to the previously identified albicidin biosynthetic gene cluster mutations, four new Tn5 mutants were identified that produced no or very few leaf symptoms. These mutants produced albicidin in vitro but did not efficiently colonize sugarcane stalks. The transposon insertion site of all four mutants was found to be located in Orf XALc_0557 of the Xa genome. This gene is predicted to encode an OmpA family outer membrane protein, a previously unrecognized and apparently essential pathogenicity factor in Xa.

Mots-clés Agrovoc : Saccharum officinarum, Xanthomonas albilineans

Mots-clés géographiques Agrovoc : Floride

Mots-clés complémentaires : Albicidine

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Rott Philippe, CIRAD-BIOS-UMR BGPI (USA) ORCID: 0000-0001-6085-6159
  • Fleites Laura, University of Florida (USA)
  • Marlow Gary C., University of Florida (USA)
  • Royer Monique, CIRAD-BIOS-UMR BGPI (FRA)
  • Gabriel Dean W., University of Florida (USA)

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