Evaluation of kahweol and cafestol in coffee tissues and roasted coffee by a new high-performance liquid chromatography methodology

Dias Rafael Carlos Eloy, Gonçalves Campanha Fernanda, Vieira Luiz Gonzaga Esteves, Pires Ferreira Lucia, Pot David, Marraccini Pierre, De Toledo Benassi Marta. 2010. Evaluation of kahweol and cafestol in coffee tissues and roasted coffee by a new high-performance liquid chromatography methodology. Journal of Agricultural and Food Chemistry, 58 (1) : pp. 88-93.

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Quartile : Outlier, Sujet : AGRICULTURE, MULTIDISCIPLINARY / Quartile : Q1, Sujet : FOOD SCIENCE & TECHNOLOGY / Quartile : Q1, Sujet : CHEMISTRY, APPLIED

Abstract : A reverse phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous quantification of kahweol and cafestol in tissues of fresh fruits, leaves, and roasted coffee beans. The best resolution was obtained with isocratic elution of acetonitrile/water (55/45% v/v) and UV detection. A single sample preparation method carried out by direct saponification and extraction with organic solvent was standardized for all matrices. Good recovery (average of 99% for kahweol and 94% for cafestol), repeatability, and linearity were obtained. Detection limits of 2.3 and 3.0 mg/100 g were observed for kahweol and cafestol. The HPLC method was effective in quantifying these diterpenes in the different coffee matrices. The endosperm and perisperm of Coffea arabica cv. IAPAR 59 showed elevated amounts of kahweol as compared to the pericarp and leaves. On the other hand, cafestol was detected in all samples except in leaves from Coffea canephora cv. Apoatã. (Résumé d'auteur)

Mots-clés Agrovoc : Fève de café, Diterpénoïde, HPLC, Coffea arabica, Coffea canephora

Mots-clés géographiques Agrovoc : Parana

Mots-clés complémentaires : Cafestol, Kahweol

Classification Agris : Q04 - Food composition
F60 - Plant physiology and biochemistry

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

  • Dias Rafael Carlos Eloy, UEL (BRA)
  • Gonçalves Campanha Fernanda, UEL (BRA)
  • Vieira Luiz Gonzaga Esteves, IAPAR (BRA)
  • Pires Ferreira Lucia, IAPAR (BRA)
  • Pot David, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0001-6144-8448
  • Marraccini Pierre, CIRAD-BIOS-UMR DAP (BRA) ORCID: 0000-0001-7637-6811
  • De Toledo Benassi Marta, UEL (BRA)

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