Gene expression variation in cocoa inoculated with endophyte

Falcão Loeni Ludke, Ramos Alessandra, Gesteira Abelmon, Argout Xavier, Cascardo Julio Cezar M., Micheli Fabienne, Marcellino Lucilia Helena. 2009. Gene expression variation in cocoa inoculated with endophyte. In : Proceedings of the 9th International Plant Molecular Biology Congress, October 25-30, 2009, St Louis, USA. by Pierre Gustafon (ed.). Lancaster : DEStech Publications, Résumé, 440. ISBN 978-1-60595-018-1 IPMB Congress. 9, St Louis, États-Unis, 25 Octobre 2009/30 Octobre 2009.

Résumé : Cocoa (Theobroma cacao L.) culture is of great socio-economic importance for Brazil. The major problem for the cocoa production in this country is the witches´ broom disease, caused by the fungus Moniliophthora perniciosa. Besides plant-pathogen interactions, important for the development of the disease, endophytic organisms might also be involved in this pathosystem and potential resistance mechanisms. In order to get insight into the molecular bases of the interaction complex "cocoa-endophytes", we analyzed gene expression and the protein profile of cocoa after inoculation with ALB629, an endophytic bacterium isolated from resistant cocoa trees. The analysis was made by macroarrays containing ca. 700 ESTs from cocoa inoculated with M. perniciosa. We found a complex pattern of differentially expressed, up- and downregulated transcripts in cocoa leaves from plants inoculated with ALB629 as compared with uninoculated controls. These differences were also mirrored in the protein profile as revealed on 2D-SDS-PAGE. We proceed now to studies aimed at the definition of the actual role of these genes in the endophyte-cocoa interaction and their putative role in plant defense.

Mots-clés Agrovoc : Theobroma cacao, endophyte, résistance aux maladies, expression des gènes, Moniliophthora

Mots-clés géographiques Agrovoc : Brésil

Mots-clés complémentaires : Moniliophthora perniciosa

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Auteurs et affiliations

• Falcão Loeni Ludke, EMBRAPA (BRA)
• Ramos Alessandra, UFPA (BRA)
• Gesteira Abelmon, UESC (BRA)
• Argout Xavier, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0002-0100-5511
• Cascardo Julio Cezar M., UESC (BRA)
• Micheli Fabienne, CIRAD-BIOS-UMR DAP (BRA)
• Marcellino Lucilia Helena, EMBRAPA (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/555756/)

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