Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry

Maraval Isabelle, Sen Kemal, Agrebi Abdelhamid, Menut Chantal, Morere Alain, Boulanger Renaud, Gay Frédéric, Mestres Christian, Günata Ziya. 2010. Quantification of 2-acetyl-1-pyrroline in rice by stable isotope dilution assay through headspace solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry. Analytica Chimica Acta, 675 (2) : 148-155.

Quartile : Q1, Sujet : CHEMISTRY, ANALYTICAL

Résumé : A new and convenient synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor compound in rice, and its ring-deuterated analog, 2-acetyl-1-d2-pyrroline (2AP-d2), was reported. A stable isotope dilution assay (SIDA), involving headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-positive chemical ionization-ion trap-tandem mass spectrometry (GC-PCI-ITMS- MS), was developed for 2AP quantification. A divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was used for HS-SPME procedure and parameters affecting analytes recovery, such as extraction time and temperature, pH and salt, were studied. The repeatability of the method (n = 10) expressed as relative standard deviation (RSD) was 11.6%. A good linearity was observed from 5.9 to 779 ng of 2AP (r2 = 0.9989). Limits of detection (LOD) and quantification (LOQ) for 2AP were 0.1 and 0.4 ng g-1 of rice, respectively. The recovery of spiked 2AP from rice matrix was almost complete. The developed method was applied to the quantification of 2AP in aerial parts and grains of scented and non-scented rice cultivars.

Mots-clés Agrovoc : riz, composé aromatique, biosynthèse, odeur, spectrométrie de masse, chromatographie, chromatographie en phase gazeuse

Mots-clés géographiques Agrovoc : Provence-Alpes-Côte d'Azur, France

Mots-clés géographiques complémentaires : Camargue

Classification Agris : Q04 - Composition des produits alimentaires
U30 - Méthodes de recherche

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

• Maraval Isabelle, CIRAD-ES-DIR (FRA)
• Sen Kemal, University of Cukurova (TUR)
• Agrebi Abdelhamid, ENSCM (FRA)
• Menut Chantal, ENSCM (FRA)
• Morere Alain, ENSCM (FRA)
• Boulanger Renaud, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0001-9396-5634
• Gay Frédéric, CIRAD-PERSYST-UPR Ecosystèmes de plantations (THA) ORCID: 0000-0002-7624-8489
• Mestres Christian, CIRAD-PERSYST-UMR Qualisud (FRA)
• Günata Ziya, UM2 (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/556668/)

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