Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens

Totté Philippe, Mather Arshad, Reslan Lina, Boublik Yan, Niang Mamadou, Du Plessis Dion H., Dedieu Laurence. 2010. Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens. Clinical and Vaccine Immunology, 17 (8) : pp. 1211-1216.

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Quartile : Q2, Sujet : MICROBIOLOGY / Quartile : Q3, Sujet : INFECTIOUS DISEASES / Quartile : Q3, Sujet : IMMUNOLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Genes of the Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC) coding for proteins capable of eliciting protective T-cell memory responses have potential for incorporation into a recombinant subunit vaccine against contagious bovine pleuropneumonia (CBPP). Here we used lymphocytes from cattle that had completely recovered from infection to screen products of MmmSC genes for recognition by CD4+ effector memory (Tem) and central memory (Tcm) T lymphocytes. Six MmmSC genes (abc, gapN, glpO, lppA, lppB, and ptsG) were expressed as histidine-tagged recombinant polypeptides, or synthetic overlapping peptides, before inclusion in proliferation and gamma interferon (IFN- ) assays. Only two MmmSC antigens, LppA and PtsG, consistently induced recall proliferation from immune CD4+ T cells and IFN- production in all animals tested. Moreover, LppA and PtsG were shown to possess epitopes recognized by both short-lived CD4+ Tem and long-lived CD4+ Tcm cells. (Résumé d'auteur)

Mots-clés Agrovoc : Péripneumonie contagieuse bovine, Mycoplasma mycoides, Identification, Bovin, Biotype, Vaccin, Antigène, Protéine, Immunologie, Immunostimulant, Gène

Mots-clés géographiques Agrovoc : Afrique au sud du Sahara

Classification Agris : L73 - Animal diseases
L10 - Animal genetics and breeding

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Totté Philippe, CIRAD-BIOS-UMR CMAEE (FRA)
  • Mather Arshad, OVI (ZAF)
  • Reslan Lina
  • Boublik Yan, CNRS (FRA)
  • Niang Mamadou, LCV (MLI)
  • Du Plessis Dion H., OVI (ZAF)
  • Dedieu Laurence, CIRAD-BIOS-UPR Bioagresseurs de pérennes (FRA) ORCID: 0000-0002-6492-3002

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