Multiplex PCR assay to discriminate four neighbouring species of the Calliptamus genus (Orthoptera: Acrididae) from France

Blanchet Elodie, Blondin Laurence, Gagnaire P.A., Foucart Antoine, Vassal Jean-Michel, Lecoq Michel. 2010. Multiplex PCR assay to discriminate four neighbouring species of the Calliptamus genus (Orthoptera: Acrididae) from France. Bulletin of Entomological Research, 100 (6) : pp. 701-706.

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Quartile : Q1, Sujet : ENTOMOLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Definition of the genus Calliptamus (Orthoptera: Acrididae) has generated many taxonomic debates. Even now, the existence of different geographical morphs hinders species determination, particularly as concerns females and larvae. Some of these species are observed in southern France and are recognized as potential pests. To circumvent problems of species identification in ecological surveys, we developed a single multiplex PCR method based on mitochondrial Cytochrome Oxydase I diagnostic polymorphisms to differentiate between the four species, Calliptamus italicus, C. wattenwylianus, C. siciliae and C. barbarus, in southern regions of France. (Résumé d'auteur)

Mots-clés Agrovoc : Calliptamus, Identification, Espèce, Biologie moléculaire, PCR, Mitochondrie, Technique analytique, Taxonomie

Mots-clés géographiques Agrovoc : France

Classification Agris : H10 - Pests of plants
U30 - Research methods
L60 - Animal taxonomy and geography

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Blanchet Elodie, CIRAD-BIOS-UMR DAP (FRA)
  • Blondin Laurence, CIRAD-BIOS-UPR Acridologie (FRA)
  • Gagnaire P.A., CNRS (FRA)
  • Foucart Antoine, CIRAD-BIOS-UPR Acridologie (FRA)
  • Vassal Jean-Michel, CIRAD-BIOS-UPR Acridologie (FRA)
  • Lecoq Michel, CIRAD-BIOS-UPR Acridologie (FRA)

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