Molecular phylogeny of Pisolithus species from Moroccan forest woodlands

Bakkali-Yakhlef Salahedine, Mousain Daniel, Duponnois Robin, Ducousso Marc, Belkouri Abdellatif, Kerdouh Benaissa, Perrineau Marie-Mathilde, Abourouh Mohamed. 2009. Molecular phylogeny of Pisolithus species from Moroccan forest woodlands. Symbiosis, 49 (3) : pp. 157-162.

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Quartile : Q4, Sujet : MICROBIOLOGY

Abstract : The phylogenetic relationships among 200 Pisolithus basidiomata collected from pine, oak, and eucalypt forests and rockrose scrubs in Morocco were investigated. Using PCR-RFLP analysis of the internal transcribed spacer (ITS) of ribosomal DNA, this collection could be divided into 5 groups, by using PCR-RFLP analysis of the internal transcribed spacer (ITS) of ribosomal DNA. The ITS of a representative basidioma of each group was sequenced, and a phylogenetic analysis was performed. The dendrogram suggests that at least five Pisolithus species are present in Morocco. Pisolithus basidiomata collected in the Pinus and Quercus forests correspond to Pisolithus arhizus and P. species 4 as previously described by Martin and colleagues in 2002. Those collected from the eucalyptus forests, under E. gomphocephala and E. camaldulensis, correspond to P. albus and P. microcarpus. Basidiomata collected from the rockrose scrubs, under Cistus crispus, C. monspeliensis or C. salviifolius, are all identified as Pisolithus species 3. Phylogenetic analyses showed that our different Pisolithus grouped well with Pisolithus specimens from other geographical origins. (Résumé d'auteur)

Mots-clés Agrovoc : Pisolithus, Ectomycorhize, Taxonomie, Phylogénie, Séquence nucléotidique, Symbiose, Arbre forestier, Quercus, Pinus, Eucalyptus, Cistus, ADN ribosomal, Provenance, Distribution géographique, PCR, RFLP, Forêt

Mots-clés géographiques Agrovoc : Maroc

Mots-clés complémentaires : Séquencage

Classification Agris : P34 - Soil biology
F62 - Plant physiology - Growth and development
F70 - Plant taxonomy and geography
P35 - Soil fertility
K10 - Forestry production

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Bakkali-Yakhlef Salahedine, CRF (MAR)
  • Mousain Daniel, INRA (FRA)
  • Duponnois Robin, IRD (SEN)
  • Ducousso Marc, CIRAD-BIOS-UMR LSTM (NCL)
  • Belkouri Abdellatif, Ecole normale supérieure (MAR)
  • Kerdouh Benaissa, CRF (MAR)
  • Perrineau Marie-Mathilde, CIRAD-BIOS-UMR LSTM (FRA)
  • Abourouh Mohamed, CRF (MAR)

Source : Cirad - Agritrop (

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