Laboratory and field investigation of a mixture of Metarhizium acridum and Neem seed oil against the Tree Locust Anacridium melanorhodon melanorhodon (Orthoptera: Acrididae)

Haroon Wail, Pagès Christine, Vassal Jean-Michel, Abdalla Mohamed Abdalla, Luong-Skovmand My-Hanh, Lecoq Michel. 2011. Laboratory and field investigation of a mixture of Metarhizium acridum and Neem seed oil against the Tree Locust Anacridium melanorhodon melanorhodon (Orthoptera: Acrididae). Biocontrol Science and Technology, 21 (3) : pp. 353-366.

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Abstract : The in vitro compatibility of Metarhizium acridum strain IMI 330189 with different concentrations of Neem seed oil Azadirachta indica A. Juss. was investigated under laboratory conditions. Water, diesel and ground nut oil dilutions of M. acridum were inoculated into semi-synthetic culture medium with Neem oil and incubated for 10 days at 20, 28, and 348C. Fungus vegetative growth and conidia production were estimated and compatibility calculated according to the in vitro classification model [T]. Field tests were also conducted during two successive years, where fourth instar nymphs of the Tree Locust, Anacridium melanorhodon melanorhodon, were sprayed with Metarhizium, Metarhizium/ Neem mixture, Neem, or Malathion, on a plantation of Acacia senegal. Samples of the treated nymphs were taken 24 h after application, placed in cages and their mortality observed daily for 3 weeks. The compatibility test revealed that at 288C, all Neem concentrations below 2% were compatible with M. acridum and concentrations of 2 and 2.5% were moderately toxic, while at 208C, 1.0% Neem was not compatible with the fungus. In the field, Metarhizium_Neem resulted in 74 and 92% mortality during two successive years compared to 64 and 83% for Metarhizium alone. These findings clearly reveal that Neem concentration on the mixture could be increased to just under 1.0% Neem without negative impact on the fungus and that mixing Metarhizium with small quantities of Neem oil accelerates locust mortality and increases efficacy. (Résumé d'auteur)

Mots-clés Agrovoc : Acrididae, Metarhizium, Azadirachta indica, Insecticide d'origine végétale, Expérimentation au champ, Expérimentation en laboratoire, Huile végétale, Lutte biologique, Ravageur des plantes, Acacia senegal

Mots-clés géographiques Agrovoc : Soudan, France

Classification Agris : H10 - Pests of plants

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Haroon Wail, University of Kordofan (SDN)
  • Pagès Christine, CIRAD-BIOS-UPR Acridologie (FRA)
  • Vassal Jean-Michel, CIRAD-BIOS-UPR Acridologie (FRA)
  • Abdalla Mohamed Abdalla, University of Kordofan (SDN)
  • Luong-Skovmand My-Hanh
  • Lecoq Michel, CIRAD-BIOS-UPR Acridologie (FRA)

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