Agritrop
Accueil

Genome-Wide association study for resistance to aerial propagation of leaf scald in sugarcane(Saccharum spp)

Rocher Solen, Daugrois Jean-Heinrich, Nibouche Samuel, Costet Laurent, Boisne-Noc Rosiane, Roques Danièle, Toubi Lyonel, D'Hont Angélique, Hoarau Jean-Yves. 2011. Genome-Wide association study for resistance to aerial propagation of leaf scald in sugarcane(Saccharum spp). In : 9th Plant Genomics European Meetings (Plant GEM), May 04-07, 2011, Istanbul, Turkey. Sabanci University. s.l. : s.n., 1 p. Plant Genomics European Meetings. 9, Istanbul, Turquie, 4 Mai 2011/7 Mai 2011.

Communication par affiche
[img]
Prévisualisation
Version publiée - Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_560674.pdf

Télécharger (252kB) | Prévisualisation

Résumé : Leaf scald (LS) is a major sugarcane disease, resulting in cane loss and sugar yield decrease when growing susceptible cultivars. This disease is caused by a xylem invading bacterium, Xanthomonas albilineans (Xa), and is mainly transmitted by aerial contamination in tropical humid climates. Cultivar resistance is the most efficient methods used to limit pathogen spread and yield loses. In order to tag alleles controlling LS resistance, a genome-wide association study was undertaken based on a survey 189 interspecific sugarcane cultivars (Saccharum spp.) representing the worldwide diversity. This panel of cultivars was phenotyped for LS resistance under natural infestation conditions, in Guadeloupe (French West Indies) in two field trials, each consisting of a complete randomized block design with 3 replications. Two successive crop cycles were analyzed for each trial. Disease Severity (DS) was calculated by measuring symptoms on 10 stalks per replication (scale 0-5 related to the amount and length of foliar necrotic lesions) with DS =[sigma]symptoms/50. The DS obtained was normalized using arc-sinus root-square transformation. BLUPs (Best Linear Unbiased Predictors) were then calculated for each cultivar. The cultivar panel was analyzed with AFLP and DArT markers resulting in 2576 polymorphic markers (AFLP + DArT). Genetic structure of the population was analyzed by a Principal Component Analysis using a subset of markers not tightly linked and a Tracy-Widom test (SASV9.2) for the significance of each component. Associations between LS resistance and each of the 2576 polymorphic markers were tested using BLUPs values in a General Linear Model (GLM) taking into account population structure using TASSEL software. Based on permutation tests, markers were declared significant at a genome-wide type 1 error of 0.05. LS phenotyping results shows that clone x crop-cycle interaction or clone x trial interaction are both highly significant compared to residual effects (P<0.001), but clone effects always appear highly significant compared to these interactions (P<0.001). Congruency of these findings with the markers detected in each trials and crop cycles (respectively 9, 7 ,14 and 12 markers detected for trial one crop cycle 2 and 3, trial 2 crop cycle 2 and 3) is discussed. Four markers were detected in both trials and shows that quantitative dissection of alleles controlling LS resistance is feasible in a humid ecology such as the one of Guadeloupe. 1600 additional markers should be tested soon for association. These should open the construction of pan-genomic-information based models designed to predict the behavior of breeding material toward Xa resistance infection to save traditional tedious indexation efforts.

Mots-clés Agrovoc : Saccharum officinarum, Xanthomonas albilineans

Mots-clés géographiques Agrovoc : Guadeloupe, France

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Auteurs et affiliations

  • Rocher Solen, CIRAD-BIOS-UMR AGAP (FRA)
  • Daugrois Jean-Heinrich, CIRAD-BIOS-UMR BGPI (GLP)
  • Nibouche Samuel, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-2675-1382
  • Costet Laurent, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-3199-2885
  • Boisne-Noc Rosiane, CIRAD-BIOS-UMR AGAP (GLP)
  • Roques Danièle, CIRAD-BIOS-UMR AGAP (GLP)
  • Toubi Lyonel, CIRAD-BIOS-UMR AGAP (GLP)
  • D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)
  • Hoarau Jean-Yves, CIRAD-BIOS-UMR AGAP (GLP) ORCID: 0000-0001-9734-4165

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/560674/)

Voir la notice (accès réservé à Agritrop) Voir la notice (accès réservé à Agritrop)

[ Page générée et mise en cache le 2024-03-28 ]