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Matita, a new retroelement from peanut : Characterization and evolutionary context in the light of the Arachis A-B genome divergence

Nielen Stephan, Vidigal Bruna S., Leal-Bertioli Soraya C.M., Ratnaparkhe Milind B., Paterson Andrew H., Garsmeur Olivier, D'Hont Angélique, Guimarães Patricia M., Bertioli David J.. 2012. Matita, a new retroelement from peanut : Characterization and evolutionary context in the light of the Arachis A-B genome divergence. Molecular Genetics and Genomics, 287 (1) : pp. 21-38.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : GENETICS & HEREDITY / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Cultivated peanut is an allotetraploid with an AB-genome. In order to learn more of the genomic structure of peanut, we characterized and studied the evolution of a retrotransposon originally isolated from a resistance gene analog (RGA)-containing bacterial artificial chromosome (BAC) clone. It is a moderate copy number Ty1- copia retrotransposon from the Bianca lineage and we named it Matita. Fluorescent in situ hybridization (FISH) experiments showed that Matita is mainly located on the distal regions of chromosome arms and is of approximately equal frequency on both A- and B-chromosomes. Its chromosome-specific hybridization pattern facilitates the identification of individual chromosomes, a useful cytogenetic tool considering that chromosomes in peanut are mostly metacentric and of similar size. Phylogenetic analysis of Matita elements, molecular dating of transposition events, and an estimation of the evolutionary divergence of the most probable A- and B-donor species suggest that Matita underwent its last major burst of transposition activity at around the same time of the A- and B-genome divergence about 3.5 million years ago. By probing BAC libraries with overgos probes for Matita, resistance gene analogues, and single- or low-copy genes, it was demonstrated that Matita is not randomly distributed in the genome but exhibits a significant tendency of being more abundant near resistance gene homologues than near single- copy genes. The described work is a further step towards broadening the knowledge on genomic and chromosomal structure of peanut and on its evolution. (Résumé d'auteur)

Mots-clés Agrovoc : Arachis, Arachis hypogaea, Évolution, Fluorescence, Phylogénie, Transposition de gènes, Génie génétique, Chromosome, Résistance génétique, génomique, Génome

Mots-clés géographiques Agrovoc : Brésil

Mots-clés complémentaires : Chromosome artificiel

Classification Agris : F30 - Plant genetics and breeding
U30 - Research methods

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Nielen Stephan, EMBRAPA (BRA)
  • Vidigal Bruna S., UNB [Universidade de Brasilia] (BRA)
  • Leal-Bertioli Soraya C.M., EMBRAPA (BRA)
  • Ratnaparkhe Milind B., University of Georgia (USA)
  • Paterson Andrew H., University of Georgia (USA)
  • Garsmeur Olivier, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8869-3689
  • D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)
  • Guimarães Patricia M., EMBRAPA (BRA)
  • Bertioli David J., UCB (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/562759/)

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