Molecular diagnosis by PCR-DHPLC technique of wood-decay fungi in historical buildings in Italy

Zaremski Alba, Palanti Sabrina, Mannucci Massimo, Gastonguay Louis, Le Floch Gaetan. 2011. Molecular diagnosis by PCR-DHPLC technique of wood-decay fungi in historical buildings in Italy. Pro Ligno, 7 (4) : 92-97.

Titre roumain; moldave : Diagnostic molecular prin tehnica PCR-DHPLC a ciupercilor ce degradeaza lemnul in cladirile istorice din Italia

Résumé : Wood inhabiting fungi cause real problems in the preservation of wooden surfaces and are responsible for the deterioration of cultural heritage. The identification of fungi based on morphological characteristics is still a topical issue. Nevertheless, they are limited for characterization and identification on an intraspecific level and even sometimes on an interspecific level. It is not always evident and thus many fungi remain unnamed or confused. The objective of this study was to circumvent these limitations by using a new molecular approach allowing fungal detection and identification in historic buildings in Italy. Fungal colonization was assessed by using PCR amplification and amplicons separation by Denaturing High Performance Liquid Chromatography. Due to its high sensitivity, the PCR-DHPLC technique was optimised to profile fungal communities in wood decay as well as ubiquitous contaminants.

Mots-clés Agrovoc : bois de charpente, PCR, diagnostic, Champignon, carie du bois, préservation du bois

Mots-clés géographiques Agrovoc : Italie

Classification Agris : N01 - Génie rural
J12 - Manutention, transport, stockage et conservation des produits forestiers
U30 - Méthodes de recherche
K50 - Technologie des produits forestiers

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

• Zaremski Alba, CIRAD-BIOS-UMR AGAP (FRA)
• Palanti Sabrina, IVALSA (ITA)
• Mannucci Massimo
• Gastonguay Louis, IREQ (CAN)
• Le Floch Gaetan, ESMISAB (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/563154/)

Métriques

[ Page générée et mise en cache le 2024-03-23 ]