Sex at the origin: an Asian population of the rice blast fungus Magnaporthe oryzae reproduces sexually

Saleh Dounia, Xu Peng, Shen Ying, Li Chengyun, Adreit Henri, Milazzo Joëlle, Ravigné Virginie, Bazin Eric, Nottéghem Jean-Loup, Fournier Elisabeth, Tharreau Didier. 2012. Sex at the origin: an Asian population of the rice blast fungus Magnaporthe oryzae reproduces sexually. Molecular Ecology, 21 (6) : pp. 1330-1344.

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Quartile : Q1, Sujet : ECOLOGY / Quartile : Q1, Sujet : EVOLUTIONARY BIOLOGY / Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Sexual reproduction may be cryptic or facultative in fungi and therefore difficult to detect. Magnaporthe oryzae, which causes blast, the most damaging fungal disease of rice, is thought to originate from southeast Asia. It reproduces asexually in all rice-growing regions. Sexual reproduction has been suspected in limited areas of southeast Asia, but has never been demonstrated in contemporary populations. We characterized several M. oryzae populations worldwide both biologically and genetically, to identify candidate populations for sexual reproduction. The sexual cycle of M. oryzae requires two strains of opposite mating types, at least one of which is female-fertile, to come into contact. In one Chinese population, the two mating types were found to be present at similar frequencies and almost all strains were female-fertile. Compatible strains from this population completed the sexual cycle in vitro and produced viable progenies. Genotypic richness and linkage disequilibrium data also supported the existence of sexual reproduction in this population. We resampled this population the following year, and the data obtained confirmed the presence of all the biological and genetic characteristics of sexual reproduction. In particular, a considerable genetic reshuffling of alleles was observed between the 2 years. Computer simulations confirmed that the observed genetic characteristics were unlikely to have arisen in the absence of recombination. We therefore concluded that a contemporary population of M. oryzae, pathogenic on rice, reproduces sexually in natura in southeast Asia. Our findings provide evidence for the loss of sexual reproduction by a fungal plant pathogen outside its centre of origin. (Résumé d'auteur)

Mots-clés Agrovoc : Magnaporthe, Reproduction sexuée, Cycle de développement, Génétique des populations, Fertilité, Recombinaison, Comportement sexuel, Oryza sativa

Mots-clés géographiques Agrovoc : Monde, Asie du Sud, France, Madagascar, États-Unis, Chine, Colombie

Mots-clés complémentaires : Magnaporthe oryzae

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Saleh Dounia, INRA (FRA)
  • Xu Peng, Yunnan Academy of Agricultural Sciences (CHN)
  • Shen Ying, CNRRI (CHN)
  • Li Chengyun, Yunnan Academy of Agricultural Sciences (CHN)
  • Adreit Henri, CIRAD-BIOS-UMR BGPI (FRA)
  • Milazzo Joëlle, CIRAD-BIOS-UMR BGPI (FRA)
  • Ravigné Virginie, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0002-4252-2574
  • Bazin Eric, Université Joseph Fourier (FRA)
  • Nottéghem Jean-Loup, Montpellier SupAgro (FRA)
  • Fournier Elisabeth, INRA (FRA)
  • Tharreau Didier, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120

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