Development of nuclear microsatellite markers for the fonio, Digitaria exilis (Poaceae), an understudied West African cereal

Barnaud Adeline, Vignes Hélène, Risterucci Ange-Marie, Noyer Jean-Louis, Pham Jean Louis, Blay Carole, Buiron Marylène, Vigouroux Yves, Billot Claire. 2012. Development of nuclear microsatellite markers for the fonio, Digitaria exilis (Poaceae), an understudied West African cereal. American Journal of Botany, 99 (3), e105-e107

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : Premise of the study: We developed nuclear microsatellite primers to explore the genetic diversity, population genetic structure, and evolutionary history of the fonio (Digitaria exilis), an understudied cereal cultivated in West Africa. o Methods and Results: We used a microsatellite-enriched library approach to isolate and characterize 38 nuclear primer pairs (31 di-, five tri-, and two tetranucleotide repeats), of which 21 were polymorphic and exhibited a clear pattern in 36 accessions from West Africa. The number of alleles per locus ranged from two to 22, with a mean of 4.71, and expected heterozygosity ranged from 0.03 to 0.93. o Conclusions: The developed set of 21 polymorphic SSR markers will provide tools for population and evolutionary genetics studies of the cultivated fonio. (Résumé d'auteur)

Mots-clés Agrovoc : Digitaria exilis, Microsatellite, Variation génétique

Mots-clés géographiques Agrovoc : Afrique occidentale, Guinée

Classification Agris : F30 - Plant genetics and breeding
F70 - Plant taxonomy and geography

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Barnaud Adeline, IRD (FRA)
  • Vignes Hélène, CIRAD-BIOS-UMR AGAP (FRA)
  • Risterucci Ange-Marie, CIRAD-BIOS-UMR AGAP (FRA)
  • Noyer Jean-Louis, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-7389-5621
  • Pham Jean Louis, IRD (FRA)
  • Blay Carole, IRD (FRA)
  • Buiron Marylène, IRD (FRA)
  • Vigouroux Yves, IRD (FRA)
  • Billot Claire, CIRAD-BIOS-UMR AGAP (FRA)

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