A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR)

Mandrou Eric, Hein Paulo Ricardo Gherardi, Villar Emilie, Vigneron Philippe, Plomion Christophe, Gion Jean-Marc. 2012. A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR). Tree Genetics and Genomes, 8 (2) : 353-364.

Quartile : Q1, Sujet : FORESTRY / Quartile : Q1, Sujet : HORTICULTURE / Quartile : Q3, Sujet : GENETICS & HEREDITY

Résumé : Lignin content and composition are considered as mandatory traits of eucalyptus breeding programs, especially for pulp, paper, and bioenergy production. In this article, we used 33 Eucalyptus urophylla full-sib families of an 8× 8 factorial design to provide estimates of genetic parameters for lignin- and growth-related traits. Secondly, from the sequencing of the 16 unrelated founders, we described the nucleotide and haplotype variability of cinnamoyl-CoA reductase (CCR), a candidate gene for lignin-related traits encoding the cinnamoyl-CoA reductase. Finally, we tested the association between CCR polymorphisms and trait variation using a mixed linear model. A high value of narrow sense heritability was obtained for lignin content (h²=0.85) and S/G ratio (h²=0.62) indicating that these traits are under strong genetic control. High levels of nucleotide (??=0.0131) and haplotype (Hd=0.958) diversity were detected for CCR. From an initial set of 152 biallelic single nucleotide polymorphisms (SNPs), a subset of 65 nonredundant loci was selected. Three intronic SNPs were found to be associated to the variation of S/G ratio after multiple testing correction. In the line of what has been obtained in forest trees, these SNPs explained between 2.45% and 2.87% of the genetic variance of the trait. This study demonstrates the interest of the candidate gene approach for quantitative trait nucleotide detection in Eucalyptus and paves the way to gene assisted selection of lignin composition in E. urophylla.

Mots-clés Agrovoc : Eucalyptus urophylla, lignine, gène, bois, oxydoréductase

Mots-clés géographiques Agrovoc : Congo

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale
K10 - Production forestière

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Mandrou Eric, INRA (FRA)
• Hein Paulo Ricardo Gherardi
• Villar Emilie, CIRAD-BIOS-UMR AGAP (FRA)
• Vigneron Philippe, CIRAD-BIOS-UMR AGAP (COG)
• Plomion Christophe, INRA (FRA)
• Gion Jean-Marc, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/563834/)

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