A reference microsatellite kit to assess for genetic diversity of Sorghum bicolor (Poaceae)

Billot Claire, Rivallan Ronan, Ndoye Sall Mbaye, Foncéka Daniel, Deu Monique, Glaszmann Jean-Christophe, Noyer Jean-Louis, Rami Jean-François, Risterucci Ange-Marie, Wincker Patrick, Ramu Punna, Hash Charles Tom. 2012. A reference microsatellite kit to assess for genetic diversity of Sorghum bicolor (Poaceae). American Journal of Botany, 99 (6), e245-e250

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : Premise of the study: Discrepancies in terms of genotyping data are frequently observed when comparing simple sequence repeat (SSR) data sets across genotyping technologies and laboratories. This technical concern introduces biases that hamper any synthetic studies or comparison of genetic diversity between collections. To prevent this for Sorghum bicolor , we developed a control kit of 48 SSR markers. Methods and Results: One hundred seventeen markers were selected along the genome to provide coverage across the length of all 10 sorghum linkage groups. They were tested for polymorphism and reproducibility across two laboratories (Centre de Cooperation Internationale en Recherche Agronomique pour le Developpement [CIRAD], France, and International Crops Research Institute for the Semi-Arid Tropics [ICRISAT], India) using two commonly used genotyping technologies (polyacrylamide gel-based technology with LI-COR sequencing machines and capillary systems with ABI sequencing apparatus) with DNA samples from a diverse set of 48 S. bicolor accessions. Conclusions: A kit for diversity analysis ( was developed. It contains information on 48 technically robust sorghum microsatellite markers and 10 DNA controls. It can further be used to calibrate sorghum SSR genotyping data acquired with different technologies and compare those to genetic diversity references. (Résumé d'auteur)

Mots-clés Agrovoc : Sorghum bicolor, Variation génétique, Marqueur génétique, Microsatellite, Génotype, Sélection, Polymorphisme, Reproduction

Mots-clés géographiques Agrovoc : Afrique, Yémen, Inde

Mots-clés complémentaires : Standardisation biologique

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Billot Claire, CIRAD-BIOS-UMR AGAP (FRA)
  • Rivallan Ronan, CIRAD-BIOS-UMR AGAP (FRA)
  • Ndoye Sall Mbaye, CERAAS (SEN)
  • Foncéka Daniel, CIRAD-BIOS-UMR AGAP (FRA)
  • Deu Monique, CIRAD-BIOS-UMR AGAP (FRA)
  • Glaszmann Jean-Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-9918-875X
  • Noyer Jean-Louis, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-7389-5621
  • Rami Jean-François, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-5679-3877
  • Risterucci Ange-Marie, CIRAD-BIOS-UMR AGAP (FRA)
  • Wincker Patrick, Institut de génomique (FRA)
  • Ramu Punna, ICRISAT (IND)
  • Hash Charles Tom, ICRISAT (IND)

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