The bacterial thiopurine methyltransferase tellurite resistance process is highly dependent upon aggregation properties and oxidative stress response

Prigent-Combaret Claire, Sanguin Hervé, Champier Ludovic, Bertrand Cédric, Monnez Claire, Colinon Céline, Blaha Didier, Ghigo Jean-Marc, Cournoyer Benoit. 2012. The bacterial thiopurine methyltransferase tellurite resistance process is highly dependent upon aggregation properties and oxidative stress response. Environmental Microbiology, 14 (10) : pp. 2645-2660.

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Quartile : Q1, Sujet : MICROBIOLOGY

Abstract : Bacterial thiopurine methyltransferases (bTPMTs) can favour resistance towards toxic tellurite oxyanions through a pathway leading to the emission of a garlic-like smell. Gene expression profiling completed by genetic, physiological and electron microscopy analyses was performed to identify key bacterial activities contributing to this resistance process. Escherichia coli strain MG1655 expressing the bTPMT was used as a cell model in these experiments. This strain produced a garlic-like smell which was found to be due to dimethyl telluride, and cell aggregates in culture media supplemented with tellurite. Properties involved in aggregation were correlated with cell attachment to polystyrene, which increased with tellurite concentrations. Gene expression profiling supported a role of adhesins in the resistance process with 14% of the tellurite-regulated genes involved in cell envelope, flagella and fimbriae biogenesis. Other tellurite-regulated genes were, at 27%, involved in energy, carbohydrate and amino acid metabolism including the synthesis of antioxidant proteins, and at 12% in the synthesis of transcriptional regulators and signal transduction systems. Escherichia coli mutants impaired in tellurite-regulated genes showed ubiquinone and adhesins synthesis, oxidative stress response, and efflux to be essential in the bTPMT resistance process. High tellurite resistance required a synergistic expression of these functions and an efficient tellurium volatilization by the bTPMT. (Résumé d'auteur)

Mots-clés Agrovoc : Bacteria, Escherichia coli, Expression des gènes, Résistance aux produits chimiques, Tellurium, Oxydation, Volatilisation, Activité enzymatique, Toxicité

Classification Agris : 000 - Other themes
P02 - Pollution

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

  • Prigent-Combaret Claire, CNRS (FRA)
  • Sanguin Hervé, CIRAD-BIOS-UMR LSTM (FRA)
  • Champier Ludovic, CNRS (FRA)
  • Bertrand Cédric, CNRS (FRA)
  • Monnez Claire, CNRS (FRA)
  • Colinon Céline, CNRS (FRA)
  • Blaha Didier, CNRS (FRA)
  • Ghigo Jean-Marc, Institut Pasteur (FRA)
  • Cournoyer Benoit, CNRS (FRA)

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