New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars

Dambier Dominique, Petit Fanny, Barantin Pascal, Ollitrault Patrick. 2015. New cybrids resulting from asexual pathway: a promise of the cybridization for creating new citrus rootstocks and cultivars. In : Proceedings of the XIIth International Citrus Congress: International Society of Citriculture. Navarro Luis (ed.), Sabater-Muñoz Beatriz (ed.), Moreno Pedro (ed.), Peña Leandro (ed.). ISHS. Leuven : ISHS, 501-510. (Acta Horticulturae, 1065) ISBN 978-94-62610-53-8 International Citrus Congress: Citrus and Health. 12, Valence, Espagne, 18 Novembre 2012/23 Novembre 2012.

Résumé : Cybridization should be a way to induce phenotypical variations in highly heterozygous secondary citrus species or genotypes, without breaking their very complex multilocus structure by sexual recombination. It should have particular interest to manipulate the nucleo-cytoplasmic male sterility, to transfer tolerance traits with cytoplasmic determinisms and eventually to improve tolerance to oxidative stress. The present work is focused on the creation of new cybrids through three different approaches of protoplast fusion: (i) symmetric hybridization, (ii) donor-recipient protoplast fusion by UV irradiation of donor protoplasts, and (iii) cytoplasts + protoplast fusion. Sixteen combinations of symmetric protoplast fusion have produced cybrids. No cybrid was obtained after UV irradiation of donor protoplasts while three combinations of cytoplasts + protoplast fusion produced cybrids. Therefore, cytoplast fusion appears much more promising than the UV approach for targeted cybridization. The innovative plant material is currently propagated for further cultivar and rootstock selection and for basic researches on the impact of nucleo-cytoplasmic interaction on desirable traits.

Classification Agris : F30 - Génétique et amélioration des plantes
F02 - Multiplication végétative des plantes

Auteurs et affiliations

• Dambier Dominique, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-9117-8853
• Petit Fanny
• Barantin Pascal, CIRAD-BIOS-UMR AGAP (FRA)
• Ollitrault Patrick, CIRAD-BIOS-UMR AGAP (ESP) ORCID: 0000-0002-9456-5517

Source : Cirad - Agritrop (https://agritrop.cirad.fr/567291/)

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