Genetic characterization of Cryptosporidium spp. in diarrhoeic children from four provinces in South Africa

Abu Samra Nada, Thompson Peter, Jori Ferran, Frean John, Poonsamy Bhavani, Du Plessis Dion H., Mogoye B., Xiao Lihua. 2013. Genetic characterization of Cryptosporidium spp. in diarrhoeic children from four provinces in South Africa. Zoonoses and Public Health, 60 (2) : pp. 154-159.

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Quartile : Q1, Sujet : VETERINARY SCIENCES / Quartile : Q3, Sujet : INFECTIOUS DISEASES

Abstract : The diversity of Cryptosporidium at species, subtype family and subtype level in diarrhoeic children was investigated in four provinces in South Africa. A total of 442 stool samples from children <5 years of age were collected under a large rotavirus surveillance programme and analysed by Ziehl-Neelsen acid-fast staining. Fifty-four (12.2%) were positive for Cryptosporidium, of which 25 were genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and DNA sequence analyses of the 18S rRNA gene. The majority of genotyped specimens were identified as C. hominis (76%), and a high genetic diversity was found with five different C. hominis subtype families (Ia, Ib, Id, Ie and If). Cryptosporidium parvum was found in 20% of the isolates, and three subtype families were identified (IIc, IIe and IIb), with subtype family IIc being the most common. One specimen was identified as C. meleagridis of the subtype family IIId. These results are in accordance with findings from other developing countries and report for the first time the presence in South Africa of C. meleagridis, various subtypes of C. parvum and the subtype family Ie of C. hominis. The results suggest that C. hominis and anthroponotic C. parvum subtypes are the major cause of cryptosporidiosis in South Africa. Further molecular studies are needed to better understand the epidemiology and public health importance of Cryptosporidium in humans in South Africa. (Résumé d'auteur)

Mots-clés Agrovoc : Cryptosporidium, Cryptosporidium parvum, Maladie de l'homme, Enfant, Diarrhée, Identification, Génotype, Biotype, Arn ribosomal, Pays en développement

Mots-clés géographiques Agrovoc : Afrique du Sud, Afrique

Mots-clés complémentaires : Cryptosporidium meleagridis

Classification Agris : L73 - Animal diseases
000 - Other themes

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Abu Samra Nada, University of Pretoria (ZAF)
  • Thompson Peter, University of Pretoria (ZAF)
  • Jori Ferran, CIRAD-ES-UPR AGIRs (ZAF) ORCID: 0000-0001-5451-7767
  • Frean John, University of the Witwatersrand (ZAF)
  • Poonsamy Bhavani, University of the Witwatersrand (ZAF)
  • Du Plessis Dion H., University of the Witwatersrand (ZAF)
  • Mogoye B., University of the Witwatersrand (ZAF)
  • Xiao Lihua, National Center for Emerging and Zoonotic Infectious Diseases (USA)

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