Emergence of atypical Mycoplasma agalactiae strains harboring a new prophage and associated with an alpine wild ungulate mortality episode

Tardy Florence, Baranowski Eric, Nouvel Laurent Xavier, Mick Virginie, Manso-Silvan Lucia, Thiaucourt François, Thebault Patricia, Breton Marc, Sirand-Pugnet Pascal, Blanchard Alain, Garnier Alexandre, Gilbert Philippe, Game Yvette, Poumarat François, Citti Christine. 2012. Emergence of atypical Mycoplasma agalactiae strains harboring a new prophage and associated with an alpine wild ungulate mortality episode. Applied and Environmental Microbiology, 78 (13) : 4659-4668.

Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : MICROBIOLOGY

Résumé : The bacterium Mycoplasma agalactiae is responsible for contagious agalactia (CA) in small domestic ruminants, a syndrome listed by the World Organization for Animal Health and responsible for severe damage to the dairy industry. Recently, we frequently isolated this pathogen from lung lesions of ibexes during a mortality episode in the French Alps. This situation was unusual in terms of host specificity and tissue tropism, raising the question of M. agalactiae emergence in wildlife. To address this issue, the ibex isolates were characterized using a combination of approaches that included antigenic profiles, molecular typing, optical mapping, and whole-genome sequencing. Genome analyses showed the presence of a new, large prophage containing 35 coding sequences (CDS) that was detected in most but not all ibex strains and has a homolog in Mycoplasma conjunctivae, a species causing keratoconjunctivitis in wild ungulates. This and the presence in all strains of large integrated conjugative elements suggested highly dynamic genomes. Nevertheless, M. agalactiae strains circulating in the ibex population were shown to be highly related, most likely originating from a single parental clone that has also spread to another wild ungulate species of the same geographical area, the chamois. These strains clearly differ from strains described in Europe so far, including those found nearby, before CA eradication a few years ago. While M. agalactiae pathogenicity in ibexes remains unclear, our data showed the emergence of atypical strains in Alpine wild ungulates, raising the question of a role for the wild fauna as a potential reservoir of pathogenic mycoplasmas.

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Tardy Florence, ANSES (FRA)
• Baranowski Eric, ENVT (FRA)
• Nouvel Laurent Xavier, ENVT (FRA)
• Mick Virginie, AFSSA (FRA)
• Manso-Silvan Lucia, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0002-5539-3804
• Thiaucourt François, CIRAD-BIOS-UMR CMAEE (FRA)
• Thebault Patricia, Université de Bordeaux II (FRA)
• Breton Marc, INRA (FRA)
• Sirand-Pugnet Pascal, INRA (FRA)
• Blanchard Alain, INRA (FRA)
• Garnier Alexandre, Parc National de la Vanoise (FRA)
• Gilbert Philippe, ONCFS (FRA)
• Game Yvette, Laboratoire Départemental d'Analyses Vétérinaires de Savoie (FRA)
• Poumarat François, ANSES (FRA)
• Citti Christine, ENVT (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/567572/)

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