Development of variable number of tandem repeats typing schemes for Ralstonia solanacearum, the agent of bacterial wilt, banana Moko disease and potato brown rot

N'Guessan Carine Aya, Brisse Sylvain, Le Roux-Nio Anne-Claire, Poussier Stéphane, Koné Daouda, Wicker Emmanuel. 2013. Development of variable number of tandem repeats typing schemes for Ralstonia solanacearum, the agent of bacterial wilt, banana Moko disease and potato brown rot. Journal of Microbiological Methods, 92 (3) : 366-374.

Quartile : Q3, Sujet : BIOCHEMICAL RESEARCH METHODS / Quartile : Q3, Sujet : MICROBIOLOGY

Résumé : Ralstonia solanacearum is an important soil borne bacterial plant pathogen causing bacterial wilt on many important crops. To better monitor epidemics, efficient tools that can identify and discriminate populations are needed. In this study, we assessed variable number of tandem repeats (VNTR) genotyping as a new tool for epidemiological surveillance of R. solanacearum phylotypes, and more specifically for the monitoring of the monomorphic ecotypes "Moko" (banana-pathogenic) and "brown rot" (potato-pathogenic under cool conditions). Screening of six R. solanacearum genome sequences lead to select 36 VNTR loci that were preliminarily amplified on 24 strains. From this step, 26 single-locus primer pairs were multiplexed, and applied to a worldwide collection of 337 strains encompassing the whole phylogenetic diversity, with revelation on a capillary-electrophoresis genotype. Four loci were monomorphic within all phylotypes and were not retained; the other loci were highly polymorphic but displayed a clear phylotype-specificity. Phylotypespecific MLVA schemes were thus defined, based on 13 loci for phylotype I, 12 loci for phylotype II, 11 loci for phylotype III and 6 for phylotype IV. MLVA typing was significantly more discriminative than egl-based sequevar typing, particularly on monomorphic "brown rot" ecotype (phylotype IIB/sequevar 1) and "Moko disease" clade 4 (Phylotype IIB/sequevar 4). Our results raise promising prospects for studies of population genetic structures and epidemiological monitoring.

Mots-clés Agrovoc : Ralstonia solanacearum, Musa, Solanum tuberosum, surveillance épidémiologique, génétique des populations, génotype, phylogénie, écotype, microsatellite

Mots-clés géographiques Agrovoc : La Réunion, France

Mots-clés complémentaires : Flétrissement bactérien

Classification Agris : H20 - Maladies des plantes
P34 - Biologie du sol

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• N'Guessan Carine Aya, Université de Cocody (CIV)
• Brisse Sylvain, Institut Pasteur (FRA)
• Le Roux-Nio Anne-Claire, Fédération nationale des producteurs de plants de pomme de terre (FRA)
• Poussier Stéphane, Université de la Réunion (REU)
• Koné Daouda, Université de Cocody (CIV)
• Wicker Emmanuel, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-0927-7404

Source : Cirad - Agritrop (https://agritrop.cirad.fr/567680/)

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