Evidence for novel hepaciviruses in rodents

Drexler Jan Felix, Corman Victor Max, Müller Marcel Alexander, Lukashev Alexander N., Gmyl Anatoly, Coutard Bruno, Adam Alexander, Ritz Daniel, Leijten Lonneke M., Van Riel Debby, Kallies Rene, Klose Stefan M., Gloza-Rausch Florian, Binger Tabea, Annan Augustina, Adu-Sarkodie Yaw, Oppong Samuel, Bourgarel Mathieu, Rupp Daniel, Hoffmann Bernd, Schlegel Mathias, Kümmerer Beate M., Krüger Detlev H., Schmidt-chanasit Jonas, Aguilar Setién Alvaro, Cottontail Veronika M., Hemachudha Thiravat, Wacharapluesadee Supaporn, Osterrieder Klaus, Bartenschlager Ralf, Matthee Sonja, Beer Martin, Kuilen Thijs, Reusken Chantal, Leroy Eric M., Ulrich Rainer G., Drosten Christian. 2013. Evidence for novel hepaciviruses in rodents. PLoS Pathogens, 9 (6):e1003438, 17 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Quartile : Outlier, Sujet : PARASITOLOGY / Quartile : Outlier, Sujet : VIROLOGY / Quartile : Outlier, Sujet : MICROBIOLOGY

Abstract : Hepatitis C virus (HCV) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. Research is complicated by a lack of accessible small animal models. The systematic investigation of viruses of small mammals could guide efforts to establish such models, while providing insight into viral evolutionary biology. We have assembled the so-far largest collection of small-mammal samples from around the world, qualified to be screened for bloodborne viruses, including sera and organs from 4,770 rodents (41 species); and sera from 2,939 bats (51 species). Three highly divergent rodent hepacivirus clades were detected in 27 (1.8%) of 1,465 European bank voles (Myodes glareolus) and 10 (1.9%) of 518 South African four-striped mice (Rhabdomys pumilio). Bats showed anti-HCV immunoblot reactivities but no virus detection, although the genetic relatedness suggested by the serologic results should have enabled RNA detection using the broadly reactive PCR assays developed for this study. 210 horses and 858 cats and dogs were tested, yielding further horseassociated hepaciviruses but none in dogs or cats. The rodent viruses were equidistant to HCV, exceeding by far the diversity of HCV and the canine/equine hepaciviruses taken together. Five full genomes were sequenced, representing all viral lineages. Salient genome features and distance criteria supported classification of all viruses as hepaciviruses. Quantitative RT-PCR, RNA in-situ hybridisation, and histopathology suggested hepatic tropism with liver inflammation resembling hepatitis C. Recombinant serology for two distinct hepacivirus lineages in 97 bank voles identified seroprevalence rates of 8.3 and 12.4%, respectively. Antibodies in bank vole sera neither cross-reacted with HCV, nor the heterologous bank vole hepacivirus. Co-occurrence of RNA and antibodies was found in 3 of 57 PCR-positive bank vole sera (5.3%). Our data enable new hypotheses regarding HCV evolution and encourage efforts to develop rodent surrogate models for HCV. (Résumé d'auteur)

Mots-clés Agrovoc : Flaviviridae, Flavivirus, Pestivirus, Hépatite, Rongeur, Mammifère, Identification, Phylogénie, Génie génétique, Génome, Distribution géographique, Chiroptera, Rodentia, Carnivora, Perissodactyla, Enquête pathologique

Mots-clés géographiques Agrovoc : Monde, Europe, Amérique centrale, Afrique occidentale, Afrique au sud du Sahara, Asie du Sud-Est, Australie

Mots-clés complémentaires : Émergence, Hepacivirus

Classification Agris : L73 - Animal diseases
L60 - Animal taxonomy and geography
L72 - Pests of animals

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Drexler Jan Felix, University of Bonn Medical Centre (DEU)
  • Corman Victor Max, University of Bonn Medical Centre (DEU)
  • Müller Marcel Alexander, University of Bonn Medical Centre (DEU)
  • Lukashev Alexander N., Chumakov Institute of Poliomyelitis and Viral Encephalitides (RUS)
  • Gmyl Anatoly, Chumakov Institute of Poliomyelitis and Viral Encephalitides (RUS)
  • Coutard Bruno, CNRS (FRA)
  • Adam Alexander, University of Cologne Medical Centre (DEU)
  • Ritz Daniel, University of Bonn Medical Centre (DEU)
  • Leijten Lonneke M., Erasmus University Rotterdam (NLD)
  • Van Riel Debby, Erasmus University Rotterdam (NLD)
  • Kallies Rene, University of Bonn Medical Centre (DEU)
  • Klose Stefan M., Université d'Ulm (DEU)
  • Gloza-Rausch Florian, University of Bonn Medical Centre (DEU)
  • Binger Tabea, University of Bonn Medical Centre (DEU)
  • Annan Augustina, KCCR (GHA)
  • Adu-Sarkodie Yaw, KNUST (GHA)
  • Oppong Samuel, KNUST (GHA)
  • Bourgarel Mathieu, CIRAD-ES-UPR AGIRs (ZWE) ORCID: 0000-0001-9774-7669
  • Rupp Daniel, University of Heidelberg (DEU)
  • Hoffmann Bernd, FLI (DEU)
  • Schlegel Mathias, Federal Research Institute for Animal Health (DEU)
  • Kümmerer Beate M., University of Bonn Medical Centre (DEU)
  • Krüger Detlev H., University of Essen (DEU)
  • Schmidt-chanasit Jonas, Bernhard Nocht Institut fuer Schiffs und Tropenkrankheiten (DEU)
  • Aguilar Setién Alvaro, Hospital de Pediatría (MEX)
  • Cottontail Veronika M., Université d'Ulm (DEU)
  • Hemachudha Thiravat, Chulalongkorn University (THA)
  • Wacharapluesadee Supaporn, Chulalongkorn University (THA)
  • Osterrieder Klaus, University of Berlin (DEU)
  • Bartenschlager Ralf, University of Heidelberg (DEU)
  • Matthee Sonja, Stellenbosch University (ZAF)
  • Beer Martin, FLI (DEU)
  • Kuilen Thijs, Erasmus University Rotterdam (NLD)
  • Reusken Chantal, Netherlands Center for Infectious Disease Control (NLD)
  • Leroy Eric M., CIRMF (GAB)
  • Ulrich Rainer G., Federal Research Institute for Animal Health (DEU)
  • Drosten Christian, University of Bonn Medical Centre (DEU)

Source : Cirad - Agritrop (

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