Isolation, characterization and cross-species amplification of polymorphic microsatellite markers for Oxytenanthera abyssinica (A. Rich.) Munro (Poaceae)

Ndiaye Aliou, Rivallan Ronan, Legavre Thierry, Brunel Dominique, Sagna Maurice, Gassama Dia Yaye Kène, Risterucci Ange-Marie. 2013. Isolation, characterization and cross-species amplification of polymorphic microsatellite markers for Oxytenanthera abyssinica (A. Rich.) Munro (Poaceae). Conservation Genetics Resources, 5 (3) : 799-802.

Quartile : Q3, Sujet : BIODIVERSITY CONSERVATION / Quartile : Q4, Sujet : GENETICS & HEREDITY

Résumé : We developed microsatellite to investigate genetic diversity of Oxytenanthera abyssinica (Bindura bamboo) a bamboo species endemic to Senegal. Using the microsatellite-enriched library method, we identified 14 primer pairs. The primers amplified 10 di-nucleotide repeats and 4 composite motifs, the number of alleles ranged from 5 to 28 with an average of 11.9. The results indicate that these primer pairs offer an appropriate amount of variation to address issues of germplasm characterization, patterns of gene flow and conservation studies. Cross amplification has been tested in other bamboo species.

Mots-clés Agrovoc : Oxytenanthera abyssinica, polymorphisme génétique, bambou, variation génétique, microsatellite

Mots-clés géographiques Agrovoc : Sénégal

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

• Ndiaye Aliou, UCAD (SEN)
• Rivallan Ronan, CIRAD-BIOS-UMR AGAP (FRA)
• Legavre Thierry, CIRAD-BIOS-UMR AGAP (FRA)
• Brunel Dominique, INRA (FRA)
• Sagna Maurice, ISRA (SEN)
• Gassama Dia Yaye Kène, UCAD (SEN)
• Risterucci Ange-Marie, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/570124/)

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