Combined genetic and genomic approaches to characterize a durable Hevea resistance to South American leaf blight : S01T07

Koop Daniela Martins, Rippel L.S., Déon Marine, Berger Angélique, Rivallan Ronan, Doare Fabien, Le Guen Vincent, Pujade-Renaud Valérie, Seguin Marc, Garcia Dominique. 2013. Combined genetic and genomic approaches to characterize a durable Hevea resistance to South American leaf blight : S01T07. In : Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century : Book of abstracts of the CIBA 2013. Brasileiro Ana Christina Miranda (ed.), Fortes Ferreira Claudia (ed.), Fernandez Diana (ed.), Micheli Fabienne (ed.), Coelho Filho M.A. (ed.), Marraccini Pierre (ed.). EMBRAPA, UESC, CIRAD, IRD. Brasilia : EMBRAPA, Résumé, pp. 14-15. Workshop on Biotic and Abiotic Stress Tolerance in Plants: the Challenge for the 21st Century, Ilhéus-Bahia, Brésil, 6 November 2013/8 November 2013.

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Abstract : The South American Leaf Blight (SALB), due to the Ascomycota Microcyclus ulei, threatens the world production of natural rubber, based on the cultivation of nearly only one species: the tropical tree Hevea brasiliensis. In connection with a breeding program, conducted in Brazil by Michelin and Cirad and aiming to create resistant cultivars of rubber tree, the genetic determinism of three sources of natural resistance to SALB has been analyzed. We developed a classical approach of QTL mapping on a segregating population issued from a crosses implying a quantitative and durable resistant in MDF180 genotype (Le Guen et al., 2008), a quantitative and by-passed resistant in Fx3899 genotype, and a qualitative resistance in Fx2784 genotype. Disease resistance of the progenies was assessed under natural infestation in field trials, in Brazil and French Guyana, or under controlled inoculations with isolated strains of Microcyclus ulei. Genetic mapping revealed unexpected genetic determinisms underlying the observed resistances: whereas a complex genetic determinism implying major resistance genes and several minor QTLs can be bypassed by the pathogen (Le Guen et al. 2007), a durable partial resistance appeared to be governed by only 2 major resistance loci (Le Guen et al. 2011). To initiate a comprehensive approach of these resistances, eleven cDNA libraries were built, sequenced and annotated from MDF180, Fx3899, PB314 (susceptible) and PB260 (susceptible) genotypes (Garcia et al., 2011). We sequenced 20,493 expressed sequence tags (EST), developed array expression analysis and identified of 212 candidate genes differentially regulated in MDF180 and 30 genes in Fx3899 during the infection process. The integrated analysis of gene expression and systems biology allowed designing a general scheme of major mechanisms associated with durable resistance of MDF180 genotype and susceptibility of PB314 genotype to South American leaf blight. Concomitantly, transcriptomic NGS data give us the possibility to analyze large data set of inoculated and non- inoculated leaves of 3 Hevea species, H. brasiliensis, H. pauciflora and H. bentamiana. Comparison of the genes differentially expressed (inoculated vs non-inoculated) indicated few common genes involved in the response to SALB between the genera of Hevea. These results, in accordance with genetic mapping, constitute a favorable context for developing a strategy of pyramiding of the resistance. Work supported by ANR, Michelin, FAPESB, CNPq. (Texte intégral)

Classification Agris : F30 - Plant genetics and breeding
H20 - Plant diseases

Auteurs et affiliations

  • Koop Daniela Martins, UESC (BRA)
  • Rippel L.S.
  • Déon Marine
  • Berger Angélique, CIRAD-BIOS-UMR AGAP (FRA)
  • Rivallan Ronan, CIRAD-BIOS-UMR AGAP (FRA)
  • Doare Fabien, CIRAD-BIOS-UPR Bioagresseurs (GUF)
  • Le Guen Vincent, CIRAD-BIOS-UMR AGAP (FRA)
  • Pujade-Renaud Valérie, CIRAD-BIOS-UMR AGAP (FRA)
  • Seguin Marc, CIRAD-BIOS-UMR AGAP (FRA)
  • Garcia Dominique, CIRAD-BIOS-UMR AGAP (FRA)

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