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Breaking dogmas: the plant vascular pathogen Xanthomonas albilineans is able to invade non-vascular tissues despite its reduced genome

Mensi Imène, Vernerey Marie-Stéphanie, Gargani Daniel, Nicole Michel, Rott Philippe. 2014. Breaking dogmas: the plant vascular pathogen Xanthomonas albilineans is able to invade non-vascular tissues despite its reduced genome. Open Biology, 4 (130116), 13 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Xanthomonas albilineans, the causal agent of sugarcane leaf scald, is missing the Hrp type III secretion system that is used by many Gram-negative bacteria to colonize their host. Until now, this pathogen was considered as strictly limited to the xylem of sugarcane. We used confocal laser scanning microscopy, immunocytochemistry and transmission electron microscopy (TEM) to investigate the localization of X. albilineans in diseased sugarcane. Sugarcane plants were inoculated with strains of the pathogen labelled with a green fluorescent protein. Confocal microscopy observations of symptomatic leaves confirmed the presence of the pathogen in the protoxylem and metaxylem; however, X. albilineans was also observed in phloem, parenchyma and bulliform cells of the infected leaves. Similarly, vascular bundles of infected sugarcane stalks were invaded by X. albilineans. Surprisingly, the pathogen was also observed in apparently intact storage cells of the stalk and in intercellular spaces between these cells. Most of these observations made by confocal microscopy were confirmed by TEM. The pathogen exits the xylem following cell wall and middle lamellae degradation, thus creating openings to reach parenchyma cells. This is the first description of a plant pathogenic vascular bacterium invading apparently intact non-vascular plant tissues and multiplying in parenchyma cells. (Résumé d'auteur)

Mots-clés Agrovoc : Saccharum officinarum, Xanthomonas albilineans, Microscopie, Laser, Immunologie, Microscopie électronique, Technique analytique, Tissu végétal, Épidémiologie

Mots-clés géographiques Agrovoc : Guadeloupe, Floride

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Mensi Imène, CIRAD-BIOS-UMR BGPI (FRA)
  • Vernerey Marie-Stéphanie, INRA (FRA)
  • Gargani Daniel, CIRAD-BIOS-UMR BGPI (FRA)
  • Nicole Michel, IRD (FRA)
  • Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159

Source : Cirad - Agritrop (https://agritrop.cirad.fr/572479/)

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