Agritrop
Home

Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.

Mérabet Chahinez, Martens M., Mahdhi Mosbah, Zakhia Frédéric, Sy Abdoulaye, Le Roux Christine, Domergue Odile, Coopman R., Bekki Abdelkader, Mars Messaoud, Willems Anne, De Lajudie Philippe. 2010. Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.. International Journal of Systematic and Evolutionary Microbiology, 60 (3) : pp. 664-674.

Journal article ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
document_574100.pdf

Télécharger (209kB)

Quartile : Q3, Sujet : MICROBIOLOGY

Abstract : Nine isolates from Argyrolobium uniflorum, Lotus creticus, Medicago sativa (Tunisia) and Lotus arabicus (Senegal) were analysed by multilocus sequence analysis (MLSA) of five housekeeping genes (recA, atpD, glnA, gltA and thrC), the 16S rRNA gene and the nodulation gene nodA. Analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, A, B and C, all belonging to the branch of the genus Ensifer, regardless of the phylogenetic reconstruction method used (maximum likelihood, maximum-parsimony, neighbour-joining). The three groups were further characterized by API 100 auxanographic tests, host specificity and nodA gene sequence analysis. On the basis of these data, clusters A and C are suggested as representing two novel species within the genus Ensifer, for which the names Ensifer numidicus sp. nov. (type strain ORS 1407T5LMG 24690T5CIP 109850T) and Ensifer garamanticus sp. nov. (type strain ORS 1400T5LMG 24692T5CIP 109916T) are proposed. The cluster B strains were assigned to Ensifer adhaerens genomovar A. (Résumé d'auteur)

Mots-clés Agrovoc : Rhizobactérie, Rhizobiaceae, Génie génétique, Taxonomie, Lotus, Medicago sativa, Séquence nucléotidique, Système racinaire

Mots-clés géographiques Agrovoc : Tunisie, Sénégal

Mots-clés complémentaires : Souche (organisme), Ensifer garamanticus, Ensifer numidicus, Ensifer adhaerens, Argyrolobium uniflorum, Lotus creticus

Classification Agris : P34 - Soil biology
F60 - Plant physiology and biochemistry
F61 - Plant physiology - Nutrition

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Mérabet Chahinez, Université d'Oran (DZA)
  • Martens M., Université de Gand (BEL)
  • Mahdhi Mosbah, Université de Gabès (TUN)
  • Zakhia Frédéric, LSTM (FRA)
  • Sy Abdoulaye, IRD (FRA)
  • Le Roux Christine, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0003-0668-3589
  • Domergue Odile, INRA (FRA)
  • Coopman R., Université de Gand (BEL)
  • Bekki Abdelkader, Université d'Oran (DZA)
  • Mars Messaoud, Université de Gabès (TUN)
  • Willems Anne, Univeriteit Gent (BEL)
  • De Lajudie Philippe, IRD (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/574100/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-04-27 ]