Nematostatic N2-fixing Crotalaria juncea for sustainable greenhouse vegetable production

Le Roux Christine, Kulagina N., Carlet Florian, Jourand Philippe, De Belder E., Caillère J., Bourrillon Julie, Ducousso Marc, Baudoin Ezékiel, Prin Yves, Tisseyre Pierre, Boursot Marc, Duponnois Robin, Galiana Antoine, De Lajudie Philippe, Deleuze F.. 2012. Nematostatic N2-fixing Crotalaria juncea for sustainable greenhouse vegetable production. In : 10th European Nitrogen Fixation Conference (ENFC), Munich, Germany - September 2-5, 2012. s.l. : s.n., Résumé, 1 p. European Nitrogen Fixation Conference. 10, Munich, Allemagne, 2 September 2012/5 September 2012.

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Abstract : We implemented complete randomised block designs over a 3-year rotation in two farm sites of southern France to test the non-crop legume Crotalaria juncea for biological control of phytoparasitic root-knot nematode populations and green manure effect in greenhouse tunnel vegetable production (lettuce) suffering from recurrent and severe nematode infestation problems. Crotalaria intercrop cultivation was introduced as an alternative cover plant to Sorghum. Crotalaria plants were inoculated at sowing with a cocktail of rhizobium strains, grown for 60-65 days, cut and the residues incorporated into soil; lettuces were then planted and harvested after 75-day growth. Several parameters were measured: Crotalaria plant growth, root nodulation, 15N natural abundance, N concentration and percentage of N2 fixed in C. juncea; subsequent lettuce crop yield and nematode infestation (galls); microbiological impact of Crotalaria in soil and roots. Soil samples were analysed for Corg-N-P contents, DNA extraction to monitor bacterial diversity, estimation of microbial catabolic potentialities (substrate induced respiration) and trap cultures of mycorrhizas. Statistical analyses show that C. juncea (1) grows rapidly in Mediterranean conditions producing a significant biomass (2) has a very positive green manure effect on lettuce production in both sites and within two successive campaigns (3) significantly reduce on lettuce gall scores. Mycorrhization index and rate of C. juncea are significantly higher than that of Ray-grass used as a reference mycorrhizogenous plant. Bacterial strains recovered from nodules show a larger diversity than that of the inoculated strains. One of the inoculated Bradyrhizobium sp. strain was still present in soil one year after inoculation. Measurements of natural abundance in 15N show that the proportion of nitrogen fixed is quite low over the whole plot (Ndfa=15%) although it varies a lot between blocks (from 3 to 30%). Nevertheless, this corresponds to about 50 Kg of fixed nitrogen per ha in 3 months. No transfer of fixed N2 from C. juncea necromass to lettuce could be detected during this 1st year of experiment. Possible remnant effect of Crotalaria rotation will be further examined in the 2nd and 3rd year of experiment. A third site of experimentation is being settled in Murcia (Spain) in 2012 (tomato production). (Texte intégral)

Classification Agris : F08 - Cropping patterns and systems
F01 - Crop husbandry
H10 - Pests of plants
F62 - Plant physiology - Growth and development
F04 - Fertilizing
P34 - Soil biology

Auteurs et affiliations

  • Le Roux Christine, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0003-0668-3589
  • Kulagina N.
  • Carlet Florian, Montpellier SupAgro (FRA)
  • Jourand Philippe, IRD (NCL)
  • De Belder E.
  • Caillère J.
  • Bourrillon Julie
  • Ducousso Marc, CIRAD-BIOS-UMR LSTM (FRA)
  • Baudoin Ezékiel, IRD (SEN)
  • Prin Yves, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0002-3706-0045
  • Tisseyre Pierre, IRD (FRA)
  • Boursot Marc, IRD (FRA)
  • Duponnois Robin, IRD (SEN)
  • Galiana Antoine, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0002-5293-5049
  • De Lajudie Philippe, IRD (FRA)
  • Deleuze F., Société Delbon (FRA)

Source : Cirad - Agritrop (

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