Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil

Majorel Clarisse, Hannibal Laure, Ducousso Marc, Lebrun Michel, Jourand Philippe. 2014. Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil. Environmental Microbiology Reports, 6 (5) : pp. 510-518.

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Quartile : Q1, Sujet : ENVIRONMENTAL SCIENCES / Quartile : Q2, Sujet : MICROBIOLOGY

Abstract : Nickel (Ni)-tolerant ectomycorrhizal Pisolithus albus was isolated from extreme ultramafic soils that are naturally rich in heavy metals. This study aimed to identify the specific molecular mechanisms associated with the response of P. albus to nickel. In presence of high concentration of nickel, P. albus Ni-tolerant isolate showed a low basal accumulation of nickel in its fungal tissues and was able to perform a metal efflux mechanism. Three genes putatively involved in metal efflux were identified from the P. albus transcriptome, and their overexpression was confirmed in the mycelium that was cultivated in vitro in the presence of nickel and in fungal tissues that were sampled in situ. Cloning these genes in yeast provided significant advantages in terms of nickel tolerance (+ 31% Ni EC50) and growth (+ 83% u) compared with controls. Furthermore, nickel efflux was also detected in the transformed yeast cells. Protein sequence analysis indicated that the genes encoded a P-type-ATPase, an ABC transporter and a major facilitator superfamily permease (MFS). This study sheds light on a global mechanism of metal efflux by P. albus cells that supports nickel tolerance. These specific responses to nickel might contribute to the fungal adaptation in ultramafic soil. (Résumé d'auteur)

Mots-clés Agrovoc : Pisolithus, Tolérance, Nickel, Physiologie, Clonage moléculaire, Biologie moléculaire, Gène, Expression des gènes, Symbiose, Mycorhizé, Type de sol chimique, Métal lourd, Ectomycorhize

Mots-clés géographiques Agrovoc : Nouvelle-Calédonie

Mots-clés complémentaires : Terrain minier, Pisolithus albus

Classification Agris : P34 - Soil biology
F30 - Plant genetics and breeding
P33 - Soil chemistry and physics
P35 - Soil fertility
P36 - Soil erosion, conservation and reclamation

Champ stratégique Cirad : Axe 6 (2014-2018) - Sociétés, natures et territoires

Auteurs et affiliations

  • Majorel Clarisse, IRD (FRA)
  • Hannibal Laure, IRD (FRA)
  • Ducousso Marc, CIRAD-BIOS-UMR LSTM (FRA)
  • Lebrun Michel, IRD (FRA)
  • Jourand Philippe, IRD (NCL)

Source : Cirad - Agritrop (

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