Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes

Silipo Alba, Vitiello Giuseppe, Gully Djamel, Sturiale Luisa, Chaintreuil Clémence, Fardoux Joël, Gargani Daniel, Lee Hae-In, Kulkarni Gargi, Busset Nicolas, Marchetti Roberta, Palmigiano Angelo, Moll Herman, Engel Regina, Lanzetta Rosa, Paduano Luigi, Parrilli Michelangelo, Chang Woo-Suk, Holst Otto, Newman Dianne K., Garozzo Domenico, D'Errico Gerardino, Giraud Eric, Molinaro Antonio. 2014. Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes. Nature Communications, 5 (5106), 11 p.

Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Lipopolysaccharides (LPSs) are major components of the outer membrane of Gram-negative bacteria and are essential for their growth and survival. They act as a structural barrier and play an important role in the interaction with eukaryotic hosts. Here we demonstrate that a photosynthetic Bradyrhizobium strain, symbiont of Aeschynomene legumes, synthesizes a unique LPS bearing a hopanoid covalently attached to lipid A. Biophysical analyses of reconstituted liposomes indicate that this hopanoid-lipid A structure reinforces the stability and rigidity of the outer membrane. In addition, the bacterium produces other hopanoid molecules not linked to LPS. A hopanoid-deficient strain, lacking a squalene hopene cyclase, displays increased sensitivity to stressful conditions and reduced ability to survive intracellularly in the host plant. This unusual combination of hopanoid and LPS molecules may represent an adaptation to optimize bacterial survival in both free-living and symbiotic states.

Mots-clés Agrovoc : Bradyrhizobium, symbiose, Aeschynomene, bactérie gram négatif, lipopolysaccharide, bactériologie, physiologie, membrane cellulaire, stress

Classification Agris : F62 - Physiologie végétale - Croissance et développement
F01 - Culture des plantes

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

• Silipo Alba, Université de Naples 2 (ITA)
• Vitiello Giuseppe, Université de Naples 2 (ITA)
• Gully Djamel, IRD (FRA)
• Sturiale Luisa, CNR (ITA)
• Chaintreuil Clémence, IRD (FRA)
• Fardoux Joël, IRD (FRA)
• Gargani Daniel, CIRAD-BIOS-UMR BGPI (FRA)
• Lee Hae-In, University of Texas (USA)
• Kulkarni Gargi, California Institute of Technology (USA)
• Busset Nicolas, IRD (FRA)
• Marchetti Roberta, Université de Naples 2 (FRA)
• Palmigiano Angelo, CNR (ITA)
• Moll Herman, Center for Medecine and Biosciences (DEU)
• Engel Regina, Center for Medecine and Biosciences (DEU)
• Lanzetta Rosa, Université de Naples 2 (ITA)
• Paduano Luigi, Université de Naples 2 (ITA)
• Parrilli Michelangelo, Université de Naples 2 (ITA)
• Chang Woo-Suk, CIRAD-BIOS-UMR LSTM (FRA)
• Holst Otto, Center for Medecine and Biosciences (DEU)
• Newman Dianne K., California Institute of Technology (USA)
• Garozzo Domenico, CNR (ITA)
• D'Errico Gerardino, Université de Naples 2 (ITA)
• Giraud Eric, IRD (FRA)
• Molinaro Antonio, Université de Naples 2 (ITA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/574331/)

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