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Sequence variation and recognition specificity of the avirulence gene AvrPiz-T in Magnaporthe Oryzae field populations

Chen Chenx, Chen Meilian, Hu Jinnan, Zhang Wenjing, Zhong Zhenhui, Jia Yulin, Allaux Ludovic, Fournier Elisabeth, Tharreau Didier, Wang Guo-Liang, Wang Zonghua, Shen Wei-Chiang, Lu Guodong, Wang Baohua, Mitchell Thomas K.. 2014. Sequence variation and recognition specificity of the avirulence gene AvrPiz-T in Magnaporthe Oryzae field populations. Fungal Genomics and Biology, 4 (1), 8 p.

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Abstract : Magnaporthe oryzae, the rice blast pathogen, causes significant annual yield loss of rice worldwide. Currently, the most effective disease control approach is deployment of host resistance through introduction of resistance (R) genes into elite cultivars. The function of each R gene relies on the specific recognition of an avirulence (AVR) gene of the pathogen. However, the introduced resistance can be broken down within a few years, due to mutation of the AVR genes in the field populations. It is known from a few case studies that AVR mutation patterns are different from one to another. Therefore, knowledge of AVR genes sequence diversity serves as fundamental background in introducing new resistance to control rice blast. In this study, we focused on a newly identified AVR gene AvrPiz-t. We PCR amplified open reading frames (ORFs) of AvrPiz-t as well as promoter regions to detect size variation at this locus in 711 isolates of M. oryzae collected from 38 countries and regions. Through sequencing and Southern hybridization of the amplified locus, strains with polymorphisms in the ORF were classified into groups based on mutation type and site. Natural selection intensity on this gene was calculated and pathogenicity assays were applied to evaluate the association between AvrPiz-t ORF/promoter polymorphism and virulence. In conclusion, sequences at the AvrPiz-t locus were revealed to contain variations at both promoter and ORF regions. This locus is undergoing a relatively strong positive selection. The diversity in coding sequence and the insertions of transposable elements in the promoter region enable M. oryzae to evade recognition by the cognate Piz-t R gene in the host. (Résumé d'auteur)

Mots-clés Agrovoc : Magnaporthe grisea, Oryza sativa, Avirulence, Résistance génétique, Résistance aux maladies, Contrôle de maladies, mécanisme de défense, Gène, Mutation, Variation génétique, Transposition de gènes, transposon, Hybridation, Polymorphisme génétique, PCR, Génie génétique, Génétique des populations

Mots-clés géographiques Agrovoc : Monde, Chine, Égypte, Inde, Philippines, Brésil, Burundi, Côte d'Ivoire, Colombie, Cameroun, République de Corée, France, Gabon, Guyane française, Hongrie, Japon, Kenya, Maroc, Madagascar, Mali, Portugal, Fédération de Russie, Rwanda, Espagne, Thaïlande, Viet Nam, États-Unis

Mots-clés complémentaires : Magnaporthe oryzae

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Chen Chenx, Fujian Agriculture and Forestry University (CHN)
  • Chen Meilian, Fujian Agriculture and Forestry University (CHN)
  • Hu Jinnan, The Ohio State University (USA)
  • Zhang Wenjing, Fujian Agriculture and Forestry University (CHN)
  • Zhong Zhenhui, Fujian Agriculture and Forestry University (CHN)
  • Jia Yulin, USDA (USA)
  • Allaux Ludovic
  • Fournier Elisabeth, INRA (FRA)
  • Tharreau Didier, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120
  • Wang Guo-Liang, The Ohio State University (USA)
  • Wang Zonghua, Fujian Agriculture and Forestry University (CHN)
  • Shen Wei-Chiang, National Taiwan University (TWN)
  • Lu Guodong, Fujian Agriculture and Forestry University (CHN)
  • Wang Baohua, Fujian Agriculture and Forestry University (CHN)
  • Mitchell Thomas K., The Ohio State University (USA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/574454/)

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