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Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants

Filloux Denis, Dallot Sylvie, Delaunay Agnès, Galzi Serge, Jacquot Emmanuel, Roumagnac Philippe. 2015. Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants. In : Plant pathology: techniques and protocols. Lacomme Christophe (ed.). New York : Springer [Etats-Unis], pp. 249-257. (Methods in Molecular Biology, 1302) ISBN 978-1-4939-2619-0

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Abstract : This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species. (Résumé d'auteur)

Classification Agris : H20 - Plant diseases
U30 - Research methods

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
  • Dallot Sylvie, INRA (FRA)
  • Delaunay Agnès, INRA (FRA)
  • Galzi Serge, CIRAD-BIOS-UMR BGPI (FRA)
  • Jacquot Emmanuel, INRA (FRA)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039

Autres liens de la publication

Source : Cirad-Agritrop (https://agritrop.cirad.fr/576056/)

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