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Protective effect of tropical highland blackberry juice (Rubus adenotrichos Schltdl.) against UVB-mediated damage in human epidermal keratinocytes and in a reconstituted skin equivalent model

Calvo-Castro Laura, Syed Deeba N., Chamcheu Jean C., Vilela Fernanda M.P., Pérez Ana Mercedes, Vaillant Fabrice, Rojas Miguel, Mukhtar Hasan. 2013. Protective effect of tropical highland blackberry juice (Rubus adenotrichos Schltdl.) against UVB-mediated damage in human epidermal keratinocytes and in a reconstituted skin equivalent model. Photochemistry and Photobiology, 89 (5) : pp. 1199-1207.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : BIOPHYSICS / Quartile : Q3, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Solar ultraviolet (UV) radiation, particularly its UVB (280–320 nm) spectrum, is the primary environmental stimulus leading to skin carcinogenesis. Several botanical species with antioxidant properties have shown photochemopreventive effects against UVB damage. Costa Rica's tropical highland blackberry (Rubus adenotrichos) contains important levels of phenolic compounds, mainly ellagitannins and anthocyanins, with strong antioxidant properties. In this study, we examined the photochemopreventive effect of R. adenotrichos blackberry juice (BBJ) on UVB-mediated responses in human epidermal keratinocytes and in a three-dimensional (3D) reconstituted normal human skin equivalent (SE). Pretreatment (2 h) and posttreatment (24 h) of normal human epidermal keratinocytes (NHEKs) with BBJ reduced UVB (25 mJ cm−2)-mediated (1) cyclobutane pyrimidine dimers (CPDs) and (2) 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) formation. Furthermore, treatment of NHEKs with BBJ increased UVB-mediated (1) poly(ADP-ribose) polymerase cleavage and (2) activation of caspases 3, 8 and 9. Thus, BBJ seems to alleviate UVB-induced effects by reducing DNA damage and increasing apoptosis of damaged cells. To establish the in vivo significance of these findings to human skin, immunohistochemistry studies were performed in a 3D SE model, where BBJ was also found to decrease CPDs formation. These data suggest that BBJ may be developed as an agent to ameliorate UV-induced skin damage. (Résumé d'auteur)

Mots-clés Agrovoc : Rubus, Jus de fruits, Rayonnement ultraviolet, Antioxydant, Composé phénolique, Peau, Dermatologie, Médicament, Souris

Mots-clés géographiques Agrovoc : Costa Rica

Mots-clés complémentaires : Rubus adenotrichos

Mots-clés libres : Cancer-cell proliferation, Nf-kappa-b, Antioxidant capacity, Signaling, Pathways, Phenolic compounds, Mouse skin, Green tea, Apoptosis, Photocarcinogenesis, Inhibition, Biochemistry and molecular biology, Biophysics

Classification Agris : Q60 - Processing of non-food or non-feed agricultural products
000 - Other themes

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

  • Calvo-Castro Laura, Instituto Tecnológico de Costa Rica (CRI)
  • Syed Deeba N., University of Wisconsin (USA)
  • Chamcheu Jean C., University of Wisconsin (USA)
  • Vilela Fernanda M.P., Universidade de São Paulo (BRA)
  • Pérez Ana Mercedes, UCR (CRI)
  • Vaillant Fabrice, CIRAD-PERSYST-UMR Qualisud (CRI) ORCID: 0000-0001-6318-1353
  • Rojas Miguel, UCR (CRI)
  • Mukhtar Hasan, Universidade de São Paulo (BRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/576652/)

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