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High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi

Holt Kathryn E., Parkhill Julian, Mazzoni Camila J., Roumagnac Philippe, Weill François-Xavier, Goodhead Ian, Rance Richard, Baker Stephen, Maskell Duncan J., Wain John, Dolecek Christiane, Achtman Mark, Dougan Gordon. 2008. High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi. Nature Genetics, 40 (8) : pp. 987-993.

Journal article ; Article de revue à facteur d'impact
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Url - éditeur : http://www.nature.com/ng/journal/v40/n8/pdf/ng.195.pdf

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Isolates of Salmonella enterica serovar Typhi (Typhi), a human-restricted bacterial pathogen that causes typhoid, show limited genetic variation. We generated whole-genome sequences for 19 Typhi isolates using 454 (Roche) and Solexa (Illumina) technologies. Isolates, including the previously sequenced CT18 and Ty2 isolates, were selected to represent major nodes in the phylogenetic tree. Comparative analysis showed little evidence of purifying selection, antigenic variation or recombination between isolates. Rather, evolution in the Typhi population seems to be characterized by ongoing loss of gene function, consistent with a small effective population size. The lack of evidence for antigenic variation driven by immune selection is in contrast to strong adaptive selection for mutations conferring antibiotic resistance in Typhi. The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers. (Résumé d'auteur)

Mots-clés Agrovoc : Salmonella, Sérotype, Typhoïde, Séquence nucléotidique, Génome, Évolution, Santé publique

Mots-clés complémentaires : Salmonella enterica, Séquencage

Classification Agris : L73 - Animal diseases
000 - Other themes

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Holt Kathryn E., Wellcome Trust Sanger Institute (GBR)
  • Parkhill Julian, Wellcome Trust Sanger Institute (GBR)
  • Mazzoni Camila J., UCC (IRL)
  • Roumagnac Philippe, Max-Planck Institut für Infektionsbiologie (DEU) ORCID: 0000-0001-5002-6039
  • Weill François-Xavier, Institut Pasteur (FRA)
  • Goodhead Ian, University of Liverpool (GBR)
  • Rance Richard, Wellcome Trust Sanger Institute (GBR)
  • Baker Stephen, Oxford University (GBR)
  • Maskell Duncan J., University of Cambridge (GBR)
  • Wain John, HPA (GBR)
  • Dolecek Christiane, Oxford University (VNM)
  • Achtman Mark, UCC (IRL)
  • Dougan Gordon, Wellcome Trust Sanger Institute (GBR)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/577147/)

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