Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation

Rodrigues Valérie, Holzmuller Philippe, Puech Carinne, Wesonga Hezron, Thiaucourt François, Manso-Silvan Lucia. 2015. Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation. PloS One, 10 (10):e0139678, 18 p.

https://doi.org/10.1371/journal.pone.0139678

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
journal.pone.0139678[1].pdf
Télécharger (3MB) | Prévisualisation

Url - jeu de données - Entrepôt autre : http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70059

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides (Mmm) , is a severe respiratory disease of cattle responsible for major economic losses in sub-Saharan Africa. Disease control relies mainly on the use of empirically attenu- ated vaccines that provide limited protection. Thus, understanding the virulence mechanisms used by Mmm as well as the role of the host immune system in disease development, persis- tence, and control is a prerequisite for the development of new, rationally designed control strategies. The aim of this study was to assess the use of whole blood transcriptome analysis to study cattle- Mmm interactions, starting by the characterization of the bovine response to Mmm infection during the acute form of the disease. For that purpose, we compared the tran- scriptome profile of whole blood from six cattle, before challenge by contact with Mmm - infected animals and at the appearance of first clinical signs, using a bovine microarray. Func- tional analysis revealed that 680 annotated genes were differentially expressed, with an over- whelming majority of down-regulated genes characterizing an immunosuppression. The main bio-functions affected were “ organismal survival ” , “ cellular development , morphology and functions ” and “ cell-to cell signaling and interactions ” . These affected functions were consis- tent with the results of previous in vitro immunological studies. However, microarray and qPCR validation results did not highlight pro-inflammatory molecules (such as TNF α , TLR2, IL-12B and IL-6), whereas inflammation is one of the most characteristic traits of acute CBPP. This global gene expression pattern may be considered as the result, in blood, of the local pulmo- nary response and the systemic events occurring during acute CBPP. Nevertheless, to under- stand the immune events occurring during disease, detailed analyses on the different immune cell subpopulations, either in vivo , at the local site, or in vitro , will be required. Whole blood tran- scriptome analysis remains an interesting approach for the identification of bio-signatures cor- relating to recovery and protection, which should facilitate the evaluation and validation of novel vaccine formulations.

Mots-clés Agrovoc : péripneumonie contagieuse bovine, expression des gènes, bovin, Mycoplasma mycoides, sang, contrôle de maladies, immunologie, maladie des animaux, immunodiagnostic

Mots-clés géographiques Agrovoc : France

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations