Multiple mechanisms of nitrate sensing by Arabidopsis nitrate transceptor NRT1.1

Bouguyon E., Brun F., Meynard Donaldo, Kubes M., Pervent M., Leran Sophie, Lacombe Benoit, Krouk G., Guiderdoni Emmanuel, Zazimalova E., Hoyerova K., Nacry Philippe, Gojon A.. 2015. Multiple mechanisms of nitrate sensing by Arabidopsis nitrate transceptor NRT1.1. Nature Plants, 1 (3):15015, 8 p.

Journal article ; Article de revue à facteur d'impact
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Abstract : In Arabidopsis the plasma membrane nitrate transceptor (transporter/receptor) NRT1.1 governs many physiological and developmental responses to nitrate. Alongside facilitating nitrate uptake, NRT1.1 regulates the expression levels of many nitrate assimilation pathway genes, modulates root system architecture, relieves seed dormancy and protects plants from ammonium toxicity. Here, we assess the functional and phenotypic consequences of point mutations in two key residues of NRT1.1 (P492 and T101). We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1, and its longer-term downregulation, at high nitrate concentrations. We also show that these mutations have differential effects on genome-wide gene expression. Our findings indicate that NRT1.1 activates four separate signalling mechanisms, which have independent structural bases in the protein. In particular, we present evidence to suggest that the phosphorylated and non-phosphorylated forms of NRT1.1 at T101 have distinct signalling functions, and that the nitrate-dependent regulation of root development depends on the phosphorylated form. Our findings add to the evidence that NRT1.1 is able to trigger independent signalling pathways in Arabidopsis in response to different environmental conditions. (Résumé d'auteur)

Mots-clés Agrovoc : Arabidopsis, Nitrate, Phosphorylation, Protéine végétale, Membrane cellulaire, Récepteurs, Biologie moléculaire, Expression des gènes, Croissance, Racine, Mutation, Phénotype, Adsorption, Absorption de substances nutritives, Physiologie végétale, Développement biologique

Mots-clés complémentaires : Transport membranaire

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry
F61 - Plant physiology - Nutrition

Champ stratégique Cirad : Hors axes (2014-2018)

Auteurs et affiliations

  • Bouguyon E., CNRS (FRA)
  • Brun F., CNRS (FRA)
  • Meynard Donaldo, CIRAD-BIOS-UMR AGAP (FRA)
  • Kubes M., ASCR (CZE)
  • Pervent M., CNRS (FRA)
  • Leran Sophie, CNRS (FRA)
  • Lacombe Benoit, CNRS (FRA)
  • Krouk G., CNRS (FRA)
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Zazimalova E., ASCR (CZE)
  • Hoyerova K., ASCR (CZE)
  • Nacry Philippe, INRA (FRA)
  • Gojon A., CNRS (FRA)

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