Identification and characterization of Anaplasma phagocytophilum proteins involved in infection of the tick vector, Ixodes scapularis

Villar Margarita, Ayllón Nieves, Kocan Katherine M., Bonzón-Kulichenko Elena, Alberdi Pilar, Blouin Edmour F., Weisheit Sabine, Mateos-Hernández Lourdes, Cabezas-Cruz Alejandro, Bell-Sakyi Lesley, Vancová Marie, Bílý Tomás, Meyer Damien, Sterba Jan, Contreras Marinela, Rudenko Nataliia, Grubhoffer Libor, Vázquez Jesús, de la Fuente José. 2015. Identification and characterization of Anaplasma phagocytophilum proteins involved in infection of the tick vector, Ixodes scapularis. PloS One, 10 (9):e0137237, 26 p.

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Anaplasma phagocytophilum is an emerging zoonotic pathogen transmitted by Ixodes scapularis that causes human granulocytic anaplasmosis. Here, a high throughput quantitative proteomics approach was used to characterize A. phagocytophilum proteome during rickettsial multiplication and identify proteins involved in infection of the tick vector, I. scapularis. The first step in this research was focused on tick cells infected with A. phagocytophilum and sampled at two time points containing 10–15% and 65–71% infected cells, respectively to identify key bacterial proteins over-represented in high percentage infected cells. The second step was focused on adult female tick guts and salivary glands infected with A. phagocytophilum to compare in vitro results with those occurring during bacterial infection in vivo. The results showed differences in the proteome of A. phagocytophilum in infected ticks with higher impact on protein synthesis and processing than on bacterial replication in tick salivary glands. These results correlated well with the developmental cycle of A. phagocytophilum, in which cells convert from an intracellular reticulated, replicative form to the nondividing infectious dense-core form. The analysis of A. phagocytophilum differentially represented proteins identified stress response (GroEL, HSP70) and surface (MSP4) proteins that were over-represented in high percentage infected tick cells and salivary glands when compared to low percentage infected cells and guts, respectively. The results demonstrated that MSP4, GroEL and HSP70 interact and bind to tick cells, thus playing a role in rickettsia-tick interactions. The most important finding of these studies is the increase in the level of certain bacterial stress response and surface proteins in A. phagocytophilum-infected tick cells and salivary glands with functional implication in tick-pathogen interactions. These results gave a new dimension to the role of these stress response and surface proteins during A. phagocytophilum infection in ticks. Characterization of Anaplasma proteome contributes information on host-pathogen interactions and provides targets for development of novel control strategies for pathogen infection and transmission.

Mots-clés Agrovoc : Anaplasma, identification, Ixodes scapularis, protéine bactérienne, protéine, relation hôte pathogène, Rickettsiales, maladie transmissible par tiques, transmission des maladies, infection, cycle de développement, méthodologie, vaccin, Anaplasma phagocytophilum

Mots-clés géographiques Agrovoc : Minnesota, Oklahoma

Mots-clés complémentaires : Protéomique

Classification Agris : L73 - Maladies des animaux
L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Villar Margarita, CSIC (ESP)
• Ayllón Nieves, CSIC (ESP)
• Kocan Katherine M., Oklahoma State University (USA)
• Bonzón-Kulichenko Elena, Centro Nacional de Investigaciones Cardiovasculares (ESP)
• Alberdi Pilar, CSIC (ESP)
• Blouin Edmour F., Center for Veterinary Health Sciences (USA)
• Weisheit Sabine, University of Edinburgh (GBR)
• Mateos-Hernández Lourdes, CSIC (ESP)
• Cabezas-Cruz Alejandro, CSIC (ESP)
• Bell-Sakyi Lesley, University of Edinburgh (GBR)
• Vancová Marie, ASCR (CZE)
• Bílý Tomás, ASCR (CZE)
• Meyer Damien, CIRAD-BIOS-UMR CMAEE (GLP) ORCID: 0000-0003-2735-176X
• Sterba Jan, ASCR (CZE)
• Contreras Marinela, CSIC (ESP)
• Rudenko Nataliia, ASCR (CZE)
• Grubhoffer Libor, ASCR (CZE)
• Vázquez Jesús, Centro Nacional de Investigaciones Cardiovasculares (ESP)
• de la Fuente José, CSIC (ESP)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/577758/)

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