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Genome-enabled phylogeographic investigation of the quarantine pathogen Ralstonia solanacearum race 3 biovar 2 and screening for sources of resistance against its Core effectors

Clarke Christopher R., Studholme David J., Hayes Byron W., Runde Brendan J., Weisberg Alexandra, Cai Rongman, Wroblewski Tadeusz, Daunay Marie-Christine, Wicker Emmanuel, Castillo Jose A., Vinatzer Boris A.. 2015. Genome-enabled phylogeographic investigation of the quarantine pathogen Ralstonia solanacearum race 3 biovar 2 and screening for sources of resistance against its Core effectors. Phytopathology, 105 (5) : pp. 597-607.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : PLANT SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Phylogeographic studies inform about routes of pathogen dissemination and are instrumental for improving import/export controls. Genomes of 17 isolates of the bacterial wilt and potato brown rot pathogen Ralstonia solanacearum race 3 biovar 2 (R3bv2), a Select Agent in the United States, were thus analyzed to get insight into the phylogeography of this pathogen. Thirteen of fourteen isolates from Europe, Africa, and Asia were found to belong to a single clonal lineage while isolates from South America were genetically diverse and tended to carry ancestral alleles at the analyzed genomic loci consistent with a South American origin of R3bv2. The R3bv2 isolates share a core repertoire of 31 type III-secreted effector genes representing excellent candidates to be targeted with resistance genes in breeding programs to develop durable disease resistance. Toward this goal, 27 R3bv2 effectors were tested in eggplant, tomato, pepper, tobacco, and lettuce for induction of a hypersensitive-like response indicative of recognition by cognate resistance receptors. Fifteen effectors, eight of them core effectors, triggered a response in one or more plant species. These genotypes may harbor resistance genes that could be identified and mapped, cloned, and expressed in tomato or potato, for which sources of genetic resistance to R3bv2 are extremely limited. (Résumé d'auteur)

Mots-clés Agrovoc : Ralstonia solanacearum, Phylogénie, Distribution géographique, Solanum lycopersicum, Solanum melongena, Nicotiana tabacum, Capsicum annuum, Lactuca sativa, Génome, Gène, Effecteur moléculaire, Résistance génétique, Amélioration des plantes

Mots-clés géographiques Agrovoc : Europe, Afrique, Asie, Amérique du Sud

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Clarke Christopher R., Virginia Tech (USA)
  • Studholme David J., Virginia Tech (USA)
  • Hayes Byron W., Virginia Tech (USA)
  • Runde Brendan J., Virginia Tech (USA)
  • Weisberg Alexandra, Virginia Tech (USA)
  • Cai Rongman, Virginia Tech (USA)
  • Wroblewski Tadeusz, UC (USA)
  • Daunay Marie-Christine, INRA (FRA)
  • Wicker Emmanuel, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-0927-7404
  • Castillo Jose A., Fundacion PROINPA (BOL)
  • Vinatzer Boris A., Virginia Tech (USA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/578135/)

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