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Rapid accumulation and low degradation: Key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci

Becker Nathalie, Rimbaud Loup, Chiroleu Frédéric, Reynaud Bernard, Thébaud Gaël, Lett Jean-Michel. 2015. Rapid accumulation and low degradation: Key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci. Scientific Reports, 5 (17696), 10 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
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Lett_Reynaud_Chiroleu_ Scientific Reports _ Rapid accumulation and low degradation TYLC virus in Bemisia tabaci_2015-5-17696.pdf

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Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Abstract : Of worldwide economic importance, Tomato yellow leaf curl virus (TYLCV, Begomovirus) is responsible for one of the most devastating plant diseases in warm and temperate regions. The DNA begomoviruses (Geminiviridae) are transmitted by the whitefly species complex Bemisia tabaci. Although geminiviruses have long been described as circulative non-propagative viruses, observations such as long persistence of TYLCV in B. tabaci raised the question of their possible replication in the vector. We monitored two major TYLCV strains, Mild (Mld) and Israel (IL), in the invasive B. tabaci Middle East-Asia Minor 1 cryptic species, during and after the viral acquisition, within two timeframes (0–144 hours or 0–20 days). TYLCV DNA was quantified using real-time PCR, and the complementary DNA strand of TYLCV involved in viral replication was specifically quantified using anchored real-time PCR. The DNA of both TYLCV strains accumulated exponentially during acquisition but remained stable after viral acquisition had stopped. Neither replication nor vertical transmission were observed. In conclusion, our quantification of the viral loads and complementary strands of both Mld and IL strains of TYLCV in B. tabaci point to an efficient accumulation and preservation mechanism, rather than to a dynamic equilibrium between replication and degradation. (Résumé d'auteur)

Mots-clés Agrovoc : Bemisia tabaci, Géminivirus enroulement jaune tomat, Transmission des maladies, Virus des végétaux, Virologie, ADN, PCR, Brassica oleracea, Solanum lycopersicum

Mots-clés géographiques Agrovoc : Israël, Asie centrale

Mots-clés complémentaires : Tomato yellow leaf curl virus

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Becker Nathalie, CNRS (FRA)
  • Rimbaud Loup, Montpellier SupAgro (FRA)
  • Chiroleu Frédéric, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-4874-5357
  • Reynaud Bernard, CIRAD-BIOS-UMR PVBMT (REU)
  • Thébaud Gaël, INRA (FRA)
  • Lett Jean-Michel, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/578612/)

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