Genetic structure analysis of strains causing citrus canker in Iran reveals the presence of two different lineages of Xanthomonas citri pv. citri pathotype A*

Pruvost Olivier, Goodarzi T., Boyer Karine, Soltaninejad H., Escalon Aline, Alavi S.M., Javegny Stéphanie, Boyer Claudine, Cottyn B., Gagnevin Lionel, Vernière Christian. 2015. Genetic structure analysis of strains causing citrus canker in Iran reveals the presence of two different lineages of Xanthomonas citri pv. citri pathotype A*. Plant Pathology, 64 (4) : pp. 776-784.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : AGRONOMY / Quartile : Q2, Sujet : PLANT SCIENCES

Abstract : West Asia has been recognized as a major centre for the diversification of Xanthomonas citri pv. citri, a citrus quarantine pathogen of considerable economic importance. However, little genotyping data is available mainly due to the paucity of microbial resources in this region. Using a comprehensive strain collection, several genotyping techniques and a pathogenicity assay, the status of strains causing Asiatic citrus canker in Iran, an internationally significant citrus-producing country, was clarified. All strains were genetically related to X. citri pv. citri pathotype A* (i.e. strains with a host range restricted to Mexican lime and related species) but not to pathotype A (i.e. strains with a wide host range among rutaceous species). The findings were based on discriminant analysis of the principal components of MLVA-31 data and were further confirmed by pathogenicity data. Two genetically, geographically and pathologically separate groups of strains in Iran were identified. One of the groups had never been previously reported anywhere in the world. A very strong genetic structure was found (RST = 0·938), consistent with their geographical isolation. Strains from these two groups also differed in terms of their type III effector repertoire. The atypical host range of one of these groups could explain why some Iranian strains had previously been mistakenly identified as pathotype A. This study suggests the absence of invasive pathotype A strains in Iran (known as DAPC 1), which account for most of the economically important outbreaks internationally.

Mots-clés Agrovoc : Xanthomonas campestris citri, Génotype, Identification, Génétique des populations, Lignée, Pouvoir pathogène, Provenance, Analyse discriminante, Épidémiologie, Saprophyte, Citrus, Xanthomonas

Mots-clés géographiques Agrovoc : Iran République islamique

Mots-clés complémentaires : Xanthomonas citri pv citri

Mots-clés libres : MLVA-14, MLVA-31, Molecular epidemiology, Saprophytic xanthomonads, Type III effector repertoire

Classification Agris : H20 - Plant diseases
U10 - Computer science, mathematics and statistics

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)
  • Goodarzi T., NIGEB (IRN)
  • Boyer Karine, CIRAD-BIOS-UMR PVBMT (REU)
  • Soltaninejad H., NIGEB (IRN)
  • Escalon Aline
  • Alavi S.M., NIGEB (IRN)
  • Javegny Stéphanie, CIRAD-BIOS-UMR PVBMT (REU)
  • Boyer Claudine, CIRAD-BIOS-UMR PVBMT (REU)
  • Cottyn B., ILVO (BEL)
  • Gagnevin Lionel, CIRAD-BIOS-UMR IPME (FRA)
  • Vernière Christian, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0002-2312-2073

Source : Cirad-Agritrop (

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