TcCYPR04, a cacao papain-like cysteine-protease detected in senescent and necrotic tissues interacts with a cystatin TcCYS4

Cardoso Thyago Hermylly Santana, Oliveira Freitas Ana Camila, Andrade Bruno Silva, Oliveira de Sousa Aurizângela, Da Silva Santiago André, Koop Daniela Martins, Gramacho Karina Peres, Alvim Fatima Cerqueira, Micheli Fabienne, Pirovani Carlos Priminho. 2015. TcCYPR04, a cacao papain-like cysteine-protease detected in senescent and necrotic tissues interacts with a cystatin TcCYS4. PloS One, 10 (12):e0144440, 20 p.

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Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Abstract : The interaction amongst papain-like cysteine-proteases (PLCP) and their substrates and inhibitors, such as cystatins, can be perceived as part of the molecular battlefield in plant-pathogen interaction. In cacao, four cystatins were identified and characterized by our group. We identified 448 proteases in cacao genome, whereof 134 were cysteine-proteases. We expressed in Escherichia coli a PLCP from cacao, named TcCYSPR04. Immunoblottings with anti-TcCYSPR04 exhibited protein increases during leaf development. Additional isoforms of TcCYSPR04 appeared in senescent leaves and cacao tissues infected by Moniliophthora perniciosa during the transition from the biotrophic to the saprophytic phase. TcCYSPR04 was induced in the apoplastic fluid of Catongo and TSH1188 cacao genotypes, susceptible and resistant to M. perniciosa, respectively, but greater intensity and additional isoforms were observed in TSH1188. The fungal protein MpNEP induced PLCP isoform expression in tobacco leaves, according to the cross reaction with anti-TcCYSPR04. Several protein isoforms were detected at 72 hours after treatment with MpNEP. We captured an active PLCP from cacao tissues, using a recombinant cacao cystatin immobilized in CNBr-Sepharose. Mass spectrometry showed that this protein corresponds to TcCYSPR04. A homology modeling was obtained for both proteins. In order to become active, TcCYSPR04 needs to lose its inhibitory domain. Molecular docking showed the physical-chemical complementarities of the interaction between the cacao enzyme and its inhibitor. We propose that TcCYSPR04 and its interactions with cacao cystatins are involved in the senescence and necrosis events related to witches' broom symptoms. This molecular interaction may be the target for future interventions to control witches' broom disease. (Résumé d'auteur)

Mots-clés Agrovoc : Theobroma cacao, Moniliophthora, Relation hôte pathogène, Mécanisme de défense cellulaire, Contrôle de maladies, Cystéine, Protéase, Physiologie végétale, Vieillissement, Nécrose, Résistance aux maladies, Nicotiana tabacum, Génétique moléculaire, Immunologie, Génome, Modèle de simulation

Mots-clés géographiques Agrovoc : Bahia

Mots-clés complémentaires : Moniliophthora perniciosa

Classification Agris : H20 - Plant diseases
F60 - Plant physiology and biochemistry
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Cardoso Thyago Hermylly Santana, UESC (BRA)
  • Oliveira Freitas Ana Camila, UESC (BRA)
  • Andrade Bruno Silva, UESB (BRA)
  • Oliveira de Sousa Aurizângela, UESC (BRA)
  • Da Silva Santiago André, UESC (BRA)
  • Koop Daniela Martins, UESC (BRA)
  • Gramacho Karina Peres, CEPEC (BRA)
  • Alvim Fatima Cerqueira, UESC (BRA)
  • Micheli Fabienne, CIRAD-BIOS-UMR AGAP (BRA)
  • Pirovani Carlos Priminho, UESC (BRA)

Source : Cirad-Agritrop (

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