Agritrop
Home

The O-carbamoyl-transferase Alb15 is responsible for the modification of albicidin

Petras Daniel, Kerwat Dennis, Pesic Alexander, Hempel Benjamin, Von Eckardstein Leonard, Semsary Siamak, Arasté Julie, Marguerettaz Mélanie, Royer Monique, Cociancich Stéphane, Süssmuth Roderich. 2016. The O-carbamoyl-transferase Alb15 is responsible for the modification of albicidin. ACS Chemical Biology, 11 (5) : pp. 1198-1204.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
acschembio.pdf

Télécharger (1MB) | Request a copy

Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Albicidin is a potent antibiotic and phytotoxin produced by Xanthomonas albilineans which targets the plant and bacterial DNA gyrase. We now report on a new albicidin derivative which is carbamoylated at the N-terminal coumaric acid by the action of the ATP-dependent O-carbamoyltransferase Alb15, present in the albicidin (alb) gene cluster. Carbamoyl-albicidin was characterized by tandem mass spectrometry from cultures of a Xanthomonas overproducer strain and the gene function confirmed by gene inactivation of alb15 in X. albilineans. Expression of alb15 in Escherichia coli and in vitro reconstitution of the carbamoyltransferase activity confirmed albicidin as the substrate. The chemical synthesis of carbamoyl-albicidin finally enabled us to assess its bioactivity by means of in vitro gyrase inhibition and antibacterial assays. Compared to albicidin, carbamoyl-albicidin showed a significantly higher inhibitory efficiency against bacterial gyrase (∼8 vs 49 nM), which identifies the carbamoyl group as an important structural feature of albicidin maturation. (Résumé d'auteur)

Mots-clés Agrovoc : Phytotoxine, Antibiotique, Xanthomonas albilineans, composé biochimique, Enzyme, Transférase, Efficacité d'utilisation, Saccharum officinarum, Biosynthèse, Synthèse chimique

Mots-clés complémentaires : Albicidine

Classification Agris : 000 - Other themes
H20 - Plant diseases
L73 - Animal diseases

Champ stratégique Cirad : Hors axes (2014-2018)

Auteurs et affiliations

  • Petras Daniel, Technische Universitaet Berlin (DEU)
  • Kerwat Dennis, Technische Universitaet Berlin (DEU)
  • Pesic Alexander, Technische Universitaet Berlin (DEU)
  • Hempel Benjamin, Technische Universitaet Berlin (DEU)
  • Von Eckardstein Leonard, Technische Universitaet Berlin (DEU)
  • Semsary Siamak, Technische Universitaet Berlin (DEU)
  • Arasté Julie
  • Marguerettaz Mélanie
  • Royer Monique, CIRAD-BIOS-UMR BGPI (FRA)
  • Cociancich Stéphane, CIRAD-BIOS-UMR BGPI (FRA)
  • Süssmuth Roderich, Technische Universitaet Berlin (DEU)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/580816/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-05-02 ]