Virome characterization of Culex pipiens in France through metagenomic analysis: a new virus discovered. P3

Gil Patricia, Rakotoarivony Ignace, L'Ambert Grégory, Marie Albane, Francés Benoit, Loire Etienne, Koual Rachid, Gutiérrez Serafin, Albina Emmanuel. 2016. Virome characterization of Culex pipiens in France through metagenomic analysis: a new virus discovered. P3. In : Programme and abstracts EPIZONE Going Viral. EPIZONE, INIA. Madrid : EPIZONE, 92. Annual Meeting EPIZONE Going Viral. 10, Madrid, Espagne, 27 Septembre 2016/29 Septembre 2016.

Matériel d'accompagnement : 1 poster

Résumé : Culex pipiens is a mosquito vector of West Nile virus (WNV), the causing agent of West Nile fever. Epizooties of WNV have taken place in France, mainly in French Camargue, and in some instances human cases have been diagnosed. However, beyond WNV, little is known on the viruses associated to Culex pipiens, above all on viruses non-pathogenic for humans or livestock. Such viruses could have a role in WNV epidemiology either limiting mosquito populations or modulating the chances of WNV infection of Culex pipiens. Since the human impact of an ecosystem usually has an influence on species diversity, we can imagine a negative impact of human activity on the diversity of viral communities. Here we describe the development of an effective methodology for exploring viral diversity using high-throughput sequencing. We present the first results of the virome of Culex pipiens from sites with two different level of human activity in Camargue, showing that our approach allows to identify new viruses and, in some cases, to obtain almost full-length viral genomes. (Texte intégral)

Mots-clés libres : West Nile virus, Culex pipiens, Virome, Metagenomics

Classification Agris : L72 - Organismes nuisibles des animaux
L73 - Maladies des animaux
000 - Autres thèmes

Auteurs et affiliations

• Gil Patricia, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• Rakotoarivony Ignace, CIRAD-BIOS-UMR CMAEE (FRA)
• L'Ambert Grégory, EID (FRA)
• Marie Albane, EID (FRA)
• Francés Benoit, EID (FRA)
• Loire Etienne, CIRAD-BIOS-UMR CMAEE (FRA)
• Koual Rachid, CIRAD-BIOS-UMR CMAEE (FRA)
• Gutiérrez Serafin, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0002-5277-7239
• Albina Emmanuel, CIRAD-BIOS-UMR CMAEE (GLP)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/581662/)

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