Association mapping of seed and disease resistance traits in Theobroma cacao L.

Motilal Lambert A., Zhang Dapeng, Mischke Sue, Meinhardt Lyndel W., Boccara Michel, Fouet Olivier, Lanaud Claire, Umaharan Pathmanathan. 2016. Association mapping of seed and disease resistance traits in Theobroma cacao L.. Planta, 244 (6) : pp. 1265-1276.

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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : Main conclusion Microsatellite and single nucleotide polymorphism markers that could be used in marker assisted breeding of cacao were identified for number of filled seeds, black pod resistance and witches' broom disease resistance. An association mapping approach was employed to identify markers for seed number and resistance to black pod and witches' broom disease (WBD) in cacao (Theobroma cacao L.). Ninety-five microsatellites (SSRs) and 775 single nucleotide polymorphisms (SNPs) were assessed on 483 unique trees in the International Cocoa Genebank Trinidad (ICGT). Linkage disequilibrium (LD) and association mapping studies were conducted to identify markers to tag the phenotypic traits. Decay of LD occurred over an average 9.3 cM for chromosomes 1–9 and 2.5 cM for chromosome 10. Marker/trait associations were generally identified based on general linear models (GLMs) that incorporated principal components from molecular information on relatedness factor. Seven markers (mTcCIR 8, 66, 126, 212; TcSNP368, 697, 1370) on chromosomes 1 and 9 were identified for number of filled seeds (NSEED). A single marker was found for black pod resistance (mTcCIR280) on chromosome 3, whereas six markers on chromosomes 4, 5, 6, 8, and 10 were detected for WBD (mTcCIR91, 183; TcSNP375, 720, 1230 and 1374). It is expected that this association mapping study in cacao would contribute to the knowledge of the genetic determinism of cocoa traits and that the markers identified herein would prove useful in marker assisted breeding of cacao. (Résumé d'auteur)

Mots-clés Agrovoc : Theobroma cacao, Résistance aux maladies, Résistance génétique, Carte génétique, Marqueur génétique, Microsatellite, Polymorphisme génétique, Équilibre génétique, Graine, Phytophthora palmivora, Moniliophthora, Amélioration des plantes, Phénotype, Génotype, Locus des caractères quantitatifs, Génétique des populations

Mots-clés géographiques Agrovoc : Trinité-et-Tobago, France

Mots-clés complémentaires : Moniliophthora perniciosa, SSR

Mots-clés libres : Black pod disease, Cocoa seeds, Linkage disequilibrium, Microsatellite markers, Single nucleotide, Polymorphism, Witches' broom disease

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Motilal Lambert A., UWI (TTO)
  • Zhang Dapeng, USDA (USA)
  • Mischke Sue, USDA (USA)
  • Meinhardt Lyndel W., USDA (USA)
  • Boccara Michel, CIRAD-BIOS-UMR AGAP (TTO)
  • Fouet Olivier, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8547-1474
  • Lanaud Claire, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6411-7310
  • Umaharan Pathmanathan, University of West Indies (TTO)

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