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Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia

Lacroix Audrey, Duong Veasna, Hul Vibol, San Sona, Davun Hull, Omaliss Keo, Chea Sokha, Hassanin Alexandre, Theppangna Watthana, Silithammavong Soubanh, Khammavong Kongsy, Singhalath Sinpakone, Greatorex Zoe, Fine Amanda E., Goldstein Tracey, Olson Sarah, Joly Damien O., Keatts Lucy, Dussart Philippe, Afelt Aneta, Frutos Roger, Buchy Philippe. 2017. Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia. Infection, Genetics and Evolution, 48 : pp. 10-18.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q3, Sujet : INFECTIOUS DISEASES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Anthropologie-Ethnologie

Abstract : South-East Asia is a hot spot for emerging zoonotic diseases, and bats have been recognized as hosts for a large number of zoonotic viruses such as Severe Acute Respiratory Syndrome (SARS), responsible for acute respiratory syndrome outbreaks. Thus, it is important to expand our knowledge of the presence of viruses in bats which could represent a risk to humans. Coronaviruses (CoVs) have been reported in bat species from Thailand, China, Indonesia, Taiwan and the Philippines. However no such work was conducted in Cambodia or Lao PDR. Between 2010 and 2013, 1965 bats were therefore sampled at interfaces with human populations in these two countries. They were tested for the presence of coronavirus by consensus reverse transcription-PCR assay. A total of 93 samples (4.7%) from 17 genera of bats tested positive. Sequence analysis revealed the presence of potentially 37 and 56 coronavirus belonging to alpha-coronavirus (αCoV) and beta-CoV (βCoV), respectively. The βCoVs group is known to include some coronaviruses highly pathogenic to human, such as SARS-CoV and MERS-CoV. All coronavirus sequences generated from frugivorous bats (family Pteropodidae) (n = 55) clustered with other bat βCoVs of lineage D, whereas one coronavirus from Pipistrellus coromandra fell in the lineage C of βCoVs which also includes the MERS-CoV. αCoVs were all detected in various genera of insectivorous bats and clustered with diverse bat αCoV sequences previously published. A closely related strain of PEDV, responsible for severe diarrhea in pigs (PEDV-CoV), was detected in 2 Myotis bats. We highlighted the presence and the high diversity of coronaviruses circulating in bats from Cambodia and Lao PDR. Three new bat genera and species were newly identified as host of coronaviruses, namely Macroglossus sp., Megaerops niphanae and Myotis horsfieldii. (Résumé d'auteur)

Mots-clés Agrovoc : Coronavirinae, Chiroptera, Génétique des populations, Variation génétique, Phylogénie, Biodiversité, Enquête pathologique, zoonose, Identification, Vecteur de maladie, Maladie de l'homme

Mots-clés géographiques Agrovoc : République démocratique populaire lao, Cambodge, Asie du Sud-Est

Mots-clés complémentaires : Pipistrellus coromandra, Macroglossus, Megaerops niphanae, Myotis horsfieldii

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Lacroix Audrey, CIRAD-BIOS-UMR INTERTRYP (FRA)
  • Duong Veasna, Institut Pasteur du Cambodge (KHM)
  • Hul Vibol, Institut Pasteur du Cambodge (KHM)
  • San Sona, NAVRI (KHM)
  • Davun Hull, NAVRI (KHM)
  • Omaliss Keo, Ministry of Agriculture, Forestry and Fisheries (Cambodge) (KHM)
  • Chea Sokha, Wildlife Conservation Society (KHM)
  • Hassanin Alexandre, MHN (FRA)
  • Theppangna Watthana, Ministry of Agriculture, Forestry and Fisheries (Cambodge) (LAO)
  • Silithammavong Soubanh, Wildlife Conservation Society (LAO)
  • Khammavong Kongsy, Wildlife Conservation Society (LAO)
  • Singhalath Sinpakone, Wildlife Conservation Society (LAO)
  • Greatorex Zoe, Wildlife Conservation Society (LAO)
  • Fine Amanda E., Wildlife Conservation Society (VNM)
  • Goldstein Tracey, Wildlife Conservation Society (VNM)
  • Olson Sarah, Wildlife Conservation Society (USA)
  • Joly Damien O., Wildlife Conservation Society (USA)
  • Keatts Lucy, Wildlife Conservation Society (KHM)
  • Dussart Philippe, Institut Pasteur du Cambodge (KHM)
  • Afelt Aneta, Warsaw agricultural University (POL)
  • Frutos Roger, CIRAD-BIOS-UMR INTERTRYP (FRA)
  • Buchy Philippe, Institut Pasteur du Cambodge (KHM)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/582692/)

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