Cook Beth S., Beddow Jessicxa G., Manso-Silvan Lucia, Maglennon Gareth A., Rycroft Andrew N.. 2016. Selective medium for culture of Mycoplasma hyopneumoniae. Veterinary Microbiology, 195 : 158-164.
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- Anglais
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Quartile : Q1, Sujet : VETERINARY SCIENCES / Quartile : Q2, Sujet : MICROBIOLOGY
Résumé : The fastidious porcine respiratory pathogen Mycoplasma hyopneumoniae has proven difficult to culture since it was first isolated in 1965. A reliable solid medium has been particularly challenging. Moreover, clinical and pathological samples often contain the fast-growing M. hyorhinis which contaminates and overgrows M. hyopneumoniae in primary culture. The aim of this study was to optimise the culture medium for recovery of M. hyopneumoniae and to devise a medium for selection of M. hyopneumoniae from clinical samples also containing M. hyorhinis. The solid medium devised by Niels Friis was improved by use of Purified agar and incorporation of DEAE-dextran. Addition of glucose or neutralization of acidity in liquid medium with NaOH did not improve the final yield of viable organisms or alter the timing of peak viability. Analysis of the relative susceptibility of M. hyopneumoniae and M. hyorhinis strains to four antimicrobials showed that M. hyopneumoniae is less susceptible than M. hyorhinis to kanamycin. This was consistent in all UK and Danish strains tested. A concentration of 2 μg/ml of kanamycin selectively inhibited the growth of all M. hyorhinis tested, while M. hyopneumoniae was able to grow. This forms the basis of an effective selective culture medium for M. hyopneumoniae.
Mots-clés Agrovoc : Mycoplasma hyopneumoniae, sélection, provenance
Classification Agris : L73 - Maladies des animaux
Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes
Auteurs et affiliations
- Cook Beth S., Royal Veterinary College (GBR)
- Beddow Jessicxa G., Royal Veterinary College (GBR)
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Manso-Silvan Lucia, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
ORCID: 0000-0002-5539-3804
- Maglennon Gareth A., Royal Veterinary College (GBR)
- Rycroft Andrew N., Royal Veterinary College (GBR)
Source : Cirad-Agritrop (https://agritrop.cirad.fr/582857/)
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